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首页> 外文期刊>BMC Genomics >Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.
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Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

机译：凋亡蛋白1、2和3基因抑制剂大鼠同系物的克隆和鉴定。

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Background Inhibitor of Apoptosis (IAP) proteins are key intrinsic regulators of apoptosis induced by a variety of triggers. We isolated the rat Inhibitor of Apoptosis genes 1, 2 and 3 and characterized their tissue distribution and expression. Results Rat iap-1 encodes a protein of 67.1 kDa with 73 % and 89.2 % homology to human and mouse iap-1 respectively. Rat iap-2 encodes a protein of 66.7 kDa with 81.6 % and 89.3 % homology to human and mouse iap-2 respectively. Rat iap-3 encodes a protein of 56.1 kDa with 89.5 % and 93.1 % homology to human and mouse iap-3 respectively. We have generated rabbit polyclonal antibodies against all three rat IAP genes. Northern and Western blot analysis detected rat IAP transcripts and proteins in majority of the tissues examined. In addition, a shorter, alternatively spliced transcript corresponding to iap-2 was found in testes. Conclusions We have identified three rat homologues of the IAP genes. The elevated expression of rat iap-1 and iap2 in testes suggests that these two genes play an important antiapoptotic role in spermatogenesis.
机译：背景凋亡抑制剂（IAP）蛋白是由多种触发物诱导的重要的细胞凋亡内在调节因子。我们分离了大鼠凋亡抑制基因1、2和3，并对其组织分布和表达进行了表征。结果大鼠iap-1编码的蛋白为67.1 kDa，与人和小鼠iap-1的同源性分别为73％和89.2％。大鼠iap-2编码66.7 kDa的蛋白质，与人和小鼠iap-2的同源性分别为81.6％和89.3％。大鼠iap-3编码的蛋白为56.1 kDa，分别与人和小鼠iap-3的同源性为89.5％和93.1％。我们已经产生了针对所有三个大鼠IAP基因的兔多克隆抗体。 Northern和Western印迹分析在所检查的大多数组织中检测到大鼠IAP转录本和蛋白质。另外，在睾丸中发现了对应于iap-2的较短的，剪接的转录本。结论我们已经鉴定出IAP基因的三个大鼠同源物。大鼠iap-1和iap2在睾丸中的高表达表明这两个基因在精子发生中起重要的抗凋亡作用。

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