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首页> 外文期刊>BMC Genomics >DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria
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DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria

机译：炭疽芽孢杆菌pXO2质粒与密切相关细菌的基因组序列之间的DNA序列保守性

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Background Complete sequencing and annotation of the 96.2 kb Bacillus anthracis plasmid, pXO2, predicted 85 open reading frames (ORFs). Bacillus cereus and Bacillus thuringiensis isolates that ranged in genomic similarity to B. anthracis, as determined by amplified fragment length polymorphism (AFLP) analysis, were examined by PCR for the presence of sequences similar to 47 pXO2 ORFs. Results The two most distantly related isolates examined, B. thuringiensis 33679 and B. thuringiensis AWO6, produced the greatest number of ORF sequences similar to pXO2; 10 detected in 33679 and 16 in AWO6. No more than two of the pXO2 ORFs were detected in any one of the remaining isolates. Dot-blot DNA hybridizations between pXO2 ORF fragments and total genomic DNA from AWO6 were consistent with the PCR assay results for this isolate and also revealed nine additional ORFs shared between these two bacteria. Sequences similar to the B. anthracis cap genes or their regulator, acpA, were not detected among any of the examined isolates. Conclusions The presence of pXO2 sequences in the other Bacillus isolates did not correlate with genomic relatedness established by AFLP analysis. The presence of pXO2 ORF sequences in other Bacillus species suggests the possibility that certain pXO2 plasmid gene functions may also be present in other closely related bacteria.
机译：背景技术96.2 kb炭疽芽孢杆菌质粒pXO2的完整测序和注解可预测85个开放阅读框（ORF）。通过PCR检测通过扩增片段长度多态性（AFLP）分析确定的蜡状芽孢杆菌和苏云金芽胞杆菌分离株的基因组相似性与炭疽芽孢杆菌相似，该序列是否存在与47 pXO2 ORF相似的序列。结果检查的两个最远相关的分离株，苏云金芽孢杆菌33679和苏云金芽孢杆菌AWO6，产生了与pXO2相似的最大数量的ORF序列。在33679中检测到10个，在AWO6中检测到16个。在其余任何一种分离物中未检测到多于两种的pXO2 ORF。 pXO2 ORF片段和来自AWO6的总基因组DNA之间的斑点印迹DNA杂交与该分离物的PCR测定结果一致，并且还揭示了这两种细菌之间共有9个其他ORF。在所有检测到的分离株中均未检测到与炭疽芽孢杆菌帽基因或其调节子acpA相似的序列。结论在其他芽孢杆菌中，pXO2序列的存在与AFLP分析所建立的基因组相关性无关。其他芽孢杆菌属物种中存在pXO2 ORF序列提示某些pXO2质粒基因功能也可能存在于其他密切相关的细菌中的可能性。

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