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首页> 外文期刊>BMC Genomics >Metagenomic profiles of free-living archaea, bacteria and small eukaryotes in coastal areas of Sichang island, Thailand
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Metagenomic profiles of free-living archaea, bacteria and small eukaryotes in coastal areas of Sichang island, Thailand

机译：泰国四厂岛沿海地区自由生存的古细菌，细菌和小真核生物的元基因组概况

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Background Tha Wang and Tham Phang coasts, though situated at similar oceanographic positions on Sichang island, Chonburi province, Thailand, are different in bay geography and amount of municipal disturbances. These affect the marine ecosystems. The study used metagenomics combined with 16S and 18S rDNA pyrosequencing to identify types and distributions of archaea, bacteria, fungi and small eukaryotes of sizes ranges 0.45 and ~30 μm. Results Following the open bay geography and minimal municipal sewages, Tham Phang coast showed the cleaner water properties, described by color, salinity, pH, conductivity and percent dissolved oxygen. The 16S and 18S rDNA metagenomic profiles for Tha Wang and Tham Phang coasts revealed many differences, highlighting by low Lennon and Yue & Clayton theta similarity indices (66.03-73.03% for 16S rDNA profiles, 2.85-25.38% for 18S rDNA profiles). For 16S rDNA, the percent compositions of species belonging to Proteobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Verrucomicrobia, Gammatimonadetes, Tenericutes, Acidobacteria, Spirochaetes, Chlamydiae, Euryarchaeota, Nitrospirae, Planctomycetes, Thermotogae and Aquificae were higher or distinctly present in Tha Wang. In Tham Phang, except Actinobacteria, the fewer number of prokaryotic species existed. For 18S rDNA, fungi represented 74.745% of the species in Tha Wang, whereas only 6.728% in Tham Phang. Basidiomycota (71.157%) and Ascomycota (3.060%) were the major phyla in Tha Wang. Indeed, Tha Wang-to-Tham Phang percent composition ratios for fungi Basidiomycota and Chytridiomycota were 1264.701 and 25.422, respectively. In Tham Phang, Brachiopoda (lamp shells) and Mollusca (snails) accounted for 80.380% of the 18S rDNA species detected, and their proportions were approximately tenfold greater than those in Tha Wang. Overall, coastal Tham Phang comprised abundant animal species. Conclusions Tha Wang contained numerous archaea, bacteria and fungi, many of which could synthesize useful biotechnology gas and enzymes that could also function in high-saline and high-temperature conditions. Tham Phang contained less abundant archaea, bacteria and fungi, and the majority of the extracted metagenomes belonged to animal kingdom. Many microorganisms in Tham Phang were essential for nutrient-recycling and pharmaceuticals, for instances, Streptomyces, Pennicilium and Saccharomyces . Together, the study provided metagenomic profiles of free-living prokaryotes and eukaryotes in coastal areas of Sichang island.
机译：背景尽管Tha Wang和Tham Phang海岸位于泰国春武里府Sichang岛上类似的海洋位置，但海湾的地理位置和市政干扰的数量却不同。这些影响海洋生态系统。该研究将宏基因组学与16S和18S rDNA焦磷酸测序相结合，以鉴定古细菌，细菌，真菌和大小为0.45至〜30μm的小真核生物的类型和分布。结果经过开阔海湾的地理环境和极少的城市污水排放，Tham Phang海岸表现出更清洁的水属性，用颜色，盐度，pH，电导率和溶解氧百分比来描述。 Tha Wang和Tham Phang海岸的16S和18S rDNA宏基因组学谱显示出许多差异，其中以Lennon和Yue＆Clayton theta相似性指数较低为突出（16S rDNA谱为66.03-73.03％，18S rDNA谱为2.85-25.38％）。对于16S rDNA，属于变形杆菌，拟杆菌，蓝藻，硬毛菌，疣状微生物，γ线虫，Tenericutes，嗜酸菌，螺旋藻，衣原体，真鳞古菌，硝化螺旋藻，单核菌，嗜热菌和拟水单胞菌的物种的百分比组成。在Tham Phang，除放线菌外，原核种数量较少。对于18S rDNA，真菌占Tha Wang物种的74.745％，而Tham Phang仅占6.728％。塔旺的主要种群为担子菌（71.157％）和子囊（3.060％）。实际上，真菌Basidiomycota和Chytridiomycota的Tha Wang-Tham Phang百分比组成比分别为1264.701和25.422。在Tham Phang，检测到的18S rDNA物种的腕足动物（灯壳）和软体动物（蜗牛）占80.380％，它们的比例大约比Tha Wang高出十倍。总体而言，沿海的唐邦拥有丰富的动物种类。结论Tha Wang含有许多古细菌，细菌和真菌，其中许多可以合成有用的生物技术气体和酶，这些酶和酶也可以在高盐和高温条件下起作用。 Tham Phang含有较少的古细菌，细菌和真菌，并且大多数提取的元基因组属于动物界。谭彭（Tham Phang）的许多微生物对于营养回收和药物至关重要，例如链霉菌，青霉菌和酿酒酵母。总之，该研究提供了四厂岛沿海地区自由生活的原核生物和真核生物的宏基因组学特征。

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