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首页> 外文期刊>BMC Genomics >Global transcriptome analysis of AtPAP2 - overexpressing Arabidopsisthaliana with elevated ATP
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Global transcriptome analysis of AtPAP2 - overexpressing Arabidopsisthaliana with elevated ATP

机译：ATP升高的AtPAP2-过量表达拟南芥的全局转录组分析。

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Background AtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status affects leaf and root transcriptomes. Results ATP and ADP levels in the OE lines are 30-50% and 20-50% higher than in the wild-type (WT) plants. Global transcriptome analyses indicated that transcriptional regulation does play a role in sucrose and starch metabolism, nitrogen, potassium and iron uptake, amino acids and secondary metabolites metabolism when there is an ample supply of energy. While the transcript abundance of genes encoding protein components of photosystem I (PS I), photosystem II (PS II) and light harvesting complex I (LHCI) were unaltered, changes in transcript abundance for genes encoding proteins of LHCII are significant. The gene expressions of most enzymes of the Calvin cycle, glycolysis and the tricarboxylic acid (TCA) cycle were unaltered, as these enzymes are known to be regulated by light/redox status or allosteric modulation by the products (e.g. citrate, ATP/ADP ratio), but not at the level of transcription. Conclusions AtPAP2 overexpression resulted in a widespread reprogramming of the transcriptome in the transgenic plants, which is characterized by changes in the carbon, nitrogen, potassium, and iron metabolism. The fast-growing AtPAP2 OE lines provide an interesting tool for studying the regulation of energy system in plant.
机译：背景AtPAP2是一种同时针对叶绿体和线粒体的紫色酸性磷酸酶。 AtPAP2的过表达（OE）系生长更快，产生的种子更多，并且叶蔗糖和葡萄糖含量更高。本研究旨在确定高能量状态如何影响叶和根转录组。结果OE系中的ATP和ADP水平分别比野生型（WT）植物高30-50％和20-50％。全局转录组分析表明，当能量充足时，转录调节确实在蔗糖和淀粉代谢，氮，钾和铁的吸收，氨基酸和次生代谢产物的代谢中起作用。虽然编码光系统I（PS I），光系统II（PS II）和光收集复合体I（LHCI）的蛋白质成分的基因的转录本丰度没有改变，但是编码LHCII的蛋白质的基因的转录本丰度变化却很大。卡尔文循环，糖酵解和三羧酸（TCA）循环中大多数酶的基因表达未改变，因为已知这些酶受光/氧化还原状态或产物的变构调节（例如柠檬酸盐，ATP / ADP比）调节），但不在转录级别上。结论AtPAP2的过表达导致转基因植物中转录组的广泛重编程，其特征在于碳，氮，钾和铁代谢的变化。快速增长的AtPAP2 OE线为研究工厂能源系统的调节提供了有趣的工具。

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