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首页> 外文期刊>BMC Genomics >RNA-Seq analysis of Citrus reticulata in the early stages of Xylella fastidiosa infection reveals auxin-related genes as a defense response
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RNA-Seq analysis of Citrus reticulata in the early stages of Xylella fastidiosa infection reveals auxin-related genes as a defense response

机译：木霉感染早期阶段的网状柑橘的RNA-Seq分析揭示了生长素相关基因作为防御反应

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Background Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (Citrus sinensis L. Osb). On the other hand, among the Citrus genus there are different sources of resistance against X. fastidiosa. For these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. To assess these genes we made use of mandarin (C. reticulata Blanco) that is known to be resistant to CVC and shares agronomical characteristics with sweet orange. Thus, we investigated the gene expression in Ponkan mandarin at one day after infection with X. fastidiosa, using RNA-seq. A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange. Results Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later. Among the induced transcripts, we identified genes encoding proteins similar to Pattern Recognition Receptors. Furthermore, many genes involved in secondary metabolism, biosynthesis and cell wall modification were upregulated as well as in synthesis of abscisic acid, jasmonic acid and auxin. Conclusions This work demonstrated that the defense response to the perception of bacteria involves cell wall modification and activation of hormone pathways, which probably lead to the induction of other defense-related genes. We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.
机译：背景技术由小木杆菌引起的杂色斑萎黄病（CVC）是最重要的柑橘病之一，并影响所有甜橙品种（Citrus sinensis L. Osb）。另一方面，在柑橘属中，有不同的抗X. fastidiosa的来源。对于这些物种，鉴定这些防御基因可能是通过育种或基因工程获得抗甜橙品种的重要一步。为了评估这些基因，我们使用了普通话（C. reticulata Blanco），该橘子已知对CVC有抗性，并与甜橙具有农艺特性。因此，我们使用RNA序列研究了X. fastidiosa感染后一天在Ponkan普通话中的基因表达。与易感的甜橙相比，一组被认为是抗性关键元素的基因被用来确认其在普通话中的调控。结果对Ponkan普通话的模拟接种和感染组织进行基因表达分析，鉴定出667个转录物被阻遏，而724个转录物则在后期被显着诱导。在诱导的转录本中，我们鉴定了编码类似于模式识别受体的蛋白质的基因。此外，许多与次级代谢，生物合成和细胞壁修饰有关的基因以及脱落酸，茉莉酸和生长素的合成均被上调。结论这项工作表明，对细菌感知的防御反应涉及细胞壁的修饰和激素途径的激活，这可能导致诱导其他防御相关基因。我们还假设生长素相关基因的诱导表明抗性植物最初将X. fastidiosa识别为坏死性病原体。

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