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首页> 外文期刊>BMC Genomics >Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection
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Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection

机译：冈比亚按蚊血细胞全基因组转录组分析揭示了细菌攻击和伯氏疟原虫感染后的病原体特异性特征

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Background The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. Results We identified 4047 genes expressed in hemocytes, using An. gambiae genome-wide microarrays. While 279 transcripts were significantly enriched in hemocytes relative to whole adult female mosquitoes, 959 transcripts exhibited immune challenge-related regulation. The global transcriptomic responses of hemocytes to challenge with different species of bacteria and/or different stages of malaria parasite infection revealed discrete, minimally overlapping, pathogen-specific signatures of infection-responsive gene expression; 105 of these represented putative immunity-related genes including anti-Plasmodium factors. Of particular interest was the specific co-regulation of various members of the Imd and JNK immune signaling pathways during malaria parasite invasion of the mosquito midgut epithelium. Conclusion Our genome-wide transcriptomic analysis of adult mosquito hemocytes reveals pathogen-specific signatures of gene regulation and identifies several novel candidate genes for future functional studies.
机译：背景冈比亚按蚊是人类疟疾的主要媒介。越来越多的证据表明，血细胞（血细胞）构成了针对细菌和疟疾寄生虫的蚊子固有免疫反应的重要组成部分。为了进一步表征血细胞在蚊子免疫中的作用，我们进行了成年雌性An的首次全基因组转录组分析。冈比亚血细胞被两种细菌和一种疟原虫感染后。结果我们使用An鉴定了在血细胞中表达的4047个基因。冈比亚全基因组微阵列。相对于整个成年雌性蚊子，血细胞中有279个转录物显着富集，而959个转录物表现出与免疫挑战相关的调节。血细胞对不同细菌种类和/或疟疾寄生虫感染不同阶段攻击的总体转录组反应揭示了感染反应性基因表达的离散，最小重叠，病原体特异性特征。其中105个代表推定的免疫相关基因，包括抗疟原虫因子。特别令人感兴趣的是在疟原虫入侵蚊子中肠上皮细胞过程中Imd和JNK免疫信号通路各个成员的特异性共同调节。结论我们对成年蚊子血细胞的全基因组转录组学分析揭示了病原体特异性的基因调控特征，并鉴定了一些新的候选基因用于未来的功能研究。

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