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首页> 外文期刊>BMC Genomics >Analysis of tarantula skeletal muscle protein sequences and identification of transcriptional isoforms
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Analysis of tarantula skeletal muscle protein sequences and identification of transcriptional isoforms

机译：狼蛛骨骼肌蛋白序列分析和转录亚型的鉴定

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Background Tarantula has been used as a model system for studying skeletal muscle structure and function, yet data on the genes expressed in tarantula muscle are lacking. Results We constructed a cDNA library from Aphonopelma sp. (Tarantula) skeletal muscle and got 2507 high-quality 5'ESTs (expressed sequence tags) from randomly picked clones. EST analysis showed 305 unigenes, among which 81 had more than 2 ESTs. Twenty abundant unigenes had matches to skeletal muscle-related genes including actin, myosin, tropomyosin, troponin-I, T and C, paramyosin, muscle LIM protein, muscle protein 20, a-actinin and tandem Ig/Fn motifs (found in giant sarcomere-related proteins). Matches to myosin light chain kinase and calponin were also identified. These results support the existence of both actin-linked and myosin-linked regulation in tarantula skeletal muscle. We have predicted full-length as well as partial cDNA sequences both experimentally and computationally for myosin heavy and light chains, actin, tropomyosin, and troponin-I, T and C, and have deduced the putative peptides. A preliminary analysis of the structural and functional properties was also carried out. Sequence similarities suggested multiple isoforms of most myofibrillar proteins, supporting the generality of multiple isoforms known from previous muscle sequence studies. This may be related to a mix of muscle fiber types. Conclusion The present study serves as a basis for defining the transcriptome of tarantula skeletal muscle, for future in vitro expression of tarantula proteins, and for interpreting structural and functional observations in this model species.
机译：背景狼蛛已被用作研究骨骼肌结构和功能的模型系统，但缺乏关于狼蛛肌肉中表达的基因的数据。结果我们从Aphonopelma sp。构建了一个cDNA文库。 （Tarantula）骨骼肌，并从随机挑选的克隆中获得了2507个高质量的5'EST（表达的序列标签）。 EST分析显示305个单基因，其中81个有2个以上的EST。二十种丰富的单基因与骨骼肌相关基因相匹配，包括肌动蛋白，肌球蛋白，原肌球蛋白，肌钙蛋白-I，T和C，副肌球蛋白，肌肉LIM蛋白，肌肉蛋白20，α-肌动蛋白和串联Ig / Fn基序（发现于巨大肌瘤相关蛋白）。还确定了与肌球蛋白轻链激酶和钙蛋白的匹配。这些结果支持在狼蛛骨骼肌中存在肌动蛋白连锁和肌球蛋白连锁调节。我们已经通过实验和计算预测了肌球蛋白重链和轻链，肌动蛋白，原肌球蛋白以及肌钙蛋白I，T和C的全长和部分cDNA序列，并推定了推定的肽。还对结构和功能特性进行了初步分析。序列相似性表明大多数肌原纤维蛋白具有多种同工型，从而支持了以往肌肉序列研究中已知的多种同工型。这可能与肌肉纤维类型的混合有关。结论本研究为定义狼蛛骨骼肌的转录组，未来狼蛛蛋白的体外表达以及解释该模型物种的结构和功能观察提供基础。

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