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首页> 外文期刊>BMC Genomics >Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
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Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes

机译：摩根氏摩根氏菌致病性相关基因的全基因组测序和鉴定

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Background The opportunistic enterobacterium, Morganella morganii , which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M. morganii was isolated during postoperative care of a cancer patient with a gallbladder stone who developed sepsis caused by bacteraemia. M. morganii is sometimes encountered in nosocomial settings and has been causally linked to catheter-associated bacteriuria, complex infections of the urinary and/or hepatobiliary tracts, wound infection, and septicaemia. M. morganii infection is associated with a high mortality rate, although most patients respond well to appropriate antibiotic therapy. To obtain insights into the genome biology of M. morganii and the mechanisms underlying its pathogenicity, we used Illumina technology to sequence the genome of the KT strain and compared its sequence with the genome sequences of related bacteria. Results The 3,826,919-bp sequence contained in 58 contigs has a GC content of 51.15% and includes 3,565 protein-coding sequences, 72 tRNA genes, and 10 rRNA genes. The pathogenicity-related genes encode determinants of drug resistance, fimbrial adhesins, an IgA protease, haemolysins, ureases, and insecticidal and apoptotic toxins as well as proteins found in flagellae, the iron acquisition system, a type-3 secretion system (T3SS), and several two-component systems. Comparison with 14 genome sequences from other members of Enterobacteriaceae revealed different degrees of similarity to several systems found in M. morganii . The most striking similarities were found in the IS4 family of transposases, insecticidal toxins, T3SS components, and proteins required for ethanolamine use ( eut operon) and cobalamin (vitamin B12) biosynthesis. The eut operon and the gene cluster for cobalamin biosynthesis are not present in the other Proteeae genomes analysed. Moreover, organisation of the 19 genes of the eut operon differs from that found in the other non- Proteeae enterobacterial genomes. Conclusions This is the first genome sequence of M. morganii , which is a clinically relevant pathogen. Comparative genome analysis revealed several pathogenicity-related genes and novel genes not found in the genomes of other members of Proteeae . Thus, the genome sequence of M. morganii provides important information concerning virulence and determinants of fitness in this pathogen.
机译：背景技术机会细菌性肠杆菌摩根氏菌（Morganella morganii）可以引起菌血症，是台湾彰化基督教医院患者临床感染的第九大最普遍原因。在患有由菌血症引起的败血症的胆囊结石的癌症患者的术后护理过程中，分离出了摩根摩根氏菌的KT菌株。摩氏支原体有时在医院环境中遇到，并与导管相关菌尿，泌尿和/或肝胆道的复杂感染，伤口感染和败血病有因果关系。尽管大多数患者对适当的抗生素治疗反应良好，但摩根氏支原体感染与高死亡率相关。为了深入了解摩根氏菌的基因组生物学及其致病机理，我们使用Illumina技术对KT菌株的基因组进行了测序，并将其序列与相关细菌的基因组序列进行了比较。结果58个重叠群中3,826,919-bp的GC含量为51.15％，包括3,565个蛋白编码序列，72个tRNA基因和10个rRNA基因。致病性相关基因编码抗药性，纤维粘附素，IgA蛋白酶，溶血素，脲酶，杀虫和凋亡毒素以及鞭毛，铁捕获系统，3型分泌系统（T3SS）中发现的蛋白质的决定因素，和几个两部分系统。与来自肠杆菌科其他成员的14个基因组序列的比较显示，与摩根莫氏菌中发现的几种系统有不同程度的相似性。在IS4家族的转座酶，杀虫毒素，T3SS组分以及乙醇胺使用（ut操纵子）和钴胺素（维生素B 12 ）生物合成所需的蛋白质中发现了最明显的相似性。所分析的其他Proteeae基因组中均不存在大肠杆菌操纵子和钴胺素生物合成的基因簇。此外，Eut操纵子的19个基因的组织与其他非Proteeae肠细菌基因组中发现的组织不同。结论这是M. morganii的第一个基因组序列，M。morganii是临床相关病原体。比较基因组分析显示了几种与致病性相关的基因和在Proteeae其他成员的基因组中找不到的新基因。因此，摩根分枝杆菌的基因组序列提供了有关该病原体的毒力和适应性决定因素的重要信息。

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