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首页> 外文期刊>BMC Genomics >Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)
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Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)

机译：热带根瘤菌CIAT 899和根瘤菌sp。的广泛寄主范围和环境适应性的基因组基础。 PRF 81，用于普通豆类（Phaseolus vulgaris L.）的接种剂

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Background Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 are α-Proteobacteria that establish nitrogen-fixing symbioses with a range of legume hosts. These strains are broadly used in commercial inoculants for application to common bean (Phaseolus vulgaris) in South America and Africa. Both strains display intrinsic resistance to several abiotic stressful conditions such as low soil pH and high temperatures, which are common in tropical environments, and to several antimicrobials, including pesticides. The genetic determinants of these interesting characteristics remain largely unknown. Results Genome sequencing revealed that CIAT 899 and PRF 81 share a highly-conserved symbiotic plasmid (pSym) that is present also in Rhizobium leucaenae CFN 299, a rhizobium displaying a similar host range. This pSym seems to have arisen by a co-integration event between two replicons. Remarkably, three distinct nodA genes were found in the pSym, a characteristic that may contribute to the broad host range of these rhizobia. Genes for biosynthesis and modulation of plant-hormone levels were also identified in the pSym. Analysis of genes involved in stress response showed that CIAT 899 and PRF 81 are well equipped to cope with low pH, high temperatures and also with oxidative and osmotic stresses. Interestingly, the genomes of CIAT 899 and PRF 81 had large numbers of genes encoding drug-efflux systems, which may explain their high resistance to antimicrobials. Genome analysis also revealed a wide array of traits that may allow these strains to be successful rhizosphere colonizers, including surface polysaccharides, uptake transporters and catabolic enzymes for nutrients, diverse iron-acquisition systems, cell wall-degrading enzymes, type I and IV pili, and novel T1SS and T5SS secreted adhesins. Conclusions Availability of the complete genome sequences of CIAT 899 and PRF 81 may be exploited in further efforts to understand the interaction of tropical rhizobia with common bean and other legume hosts.
机译：背景热带根瘤菌CIAT 899和根瘤菌sp。 PRF 81是α-Proteobacteria，可与多种豆科植物宿主建立固氮共生酶。这些菌株广泛用于商业接种剂，用于南美和非洲的普通豆类（菜豆）。两种菌株都对热带环境中常见的几种非生物胁迫条件（如低土壤pH和高温）和多种抗微生物剂（包括农药）具有内在抗性。这些有趣特征的遗传决定因素在很大程度上仍然未知。结果基因组测序表明，CIAT 899和PRF 81共有一个高度保守的共生质粒（pSym），该质粒也存在于白色根瘤菌CFN 299中，该根瘤菌显示出相似的宿主范围。该pSym似乎是由于两个复制子之间的共整合事件引起的。值得注意的是，在pSym中发现了三个不同的nodA基因，这一特征可能有助于这些根瘤菌的广泛寄主范围。在pSym中还鉴定了用于生物合成和调节植物激素水平的基因。对涉及应激反应的基因的分析表明，CIAT 899和PRF 81具备应对低pH值，高温以及氧化和渗透胁迫的能力。有趣的是，CIAT 899和PRF 81的基因组具有大量编码药物外排系统的基因，这可能解释了它们对抗菌剂的高度耐药性。基因组分析还揭示了许多特征，这些特征可能使这些菌株成为成功的根际定居者，包括表面多糖，吸收转运蛋白和营养分解代谢酶，多种铁捕获系统，细胞壁降解酶，I型和IV型菌毛，以及新型的T1SS和T5SS分泌的粘附素。结论CIAT 899和PRF 81完整基因组序列的可用性可用于进一步努力理解热带根瘤菌与普通豆类和其他豆类宿主的相互作用。

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