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首页> 外文期刊>BMC Genomics >Genome-wide modeling of complex phenotypes in Caenorhabditis elegans and Drosophila melanogaster
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Genome-wide modeling of complex phenotypes in Caenorhabditis elegans and Drosophila melanogaster

机译：秀丽隐杆线虫和黑腹果蝇复杂表型的全基因组建模。

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Background The genetic and molecular basis for many intermediate and end stage phenotypes in model systems such as C. elegans and D. melanogaster has long been known to involve pleiotropic effects and complex multigenic interactions. Gene sets are groups of genes that contribute to multiple biological or molecular phenomena. They have been used in the analysis of large molecular datasets such as microarray data, Next Generation sequencing, and other genomic datasets to reveal pleiotropic and multigenic contributions to phenotypic outcomes. Many model systems lack species specific organized phenotype based gene sets to enable high throughput analysis of large molecular datasets. Results and discussion Here, we describe two novel collections of gene sets in C. elegans and D. melanogaster that are based exclusively on genetically determined phenotypes and use a controlled phenotypic ontology. We use these collections to build genome-wide models of thousands of defined phenotypes in both model species. In addition, we demonstrate the utility of these gene sets in systems analysis and in analysis of gene expression-based molecular datasets and show how they are useful in analysis of genomic datasets connecting multigenic gene inputs to complex phenotypes. Conclusions Phenotypic based gene sets in both C. elegans and D. melanogaster are developed, characterized, and shown to be useful in the analysis of large scale species-specific genomic datasets. These phenotypic gene set collections will contribute to the understanding of complex phenotypic outcomes in these model systems.
机译：背景技术早已知道模型系统中的许多中间和晚期表型的遗传和分子基础，例如秀丽隐杆线虫和黑腹果蝇（D. melanogaster）涉及多效性效应和复杂的多基因相互作用。基因集是促成多种生物学或分子现象的基因组。它们已用于分析大分子数据集，例如微阵列数据，下一代测序和其他基因组数据集，以揭示多效性和多基因对表型结果的贡献。许多模型系统缺乏基于物种的特定有组织表型的基因集，无法对大型分子数据集进行高通量分析。结果和讨论在这里，我们描述线虫和黑腹果蝇的两个新的基因集集合，这些集合完全基于遗传确定的表型并使用受控表型本体。我们使用这些集合来建立两个模型物种中数千种定义表型的全基因组模型。此外，我们展示了这些基因集在系统分析和基于基因表达的分子数据集分析中的效用，并展示了它们在将多基因输入与复杂表型联系起来的基因组数据集分析中的有用性。结论在秀丽隐杆线虫和黑腹线虫中均建立了基于表型的基因集，对其进行了表征，并被证明可用于分析大规模物种特异性基因组数据集。这些表型基因集集合将有助于理解这些模型系统中复杂的表型结果。

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