• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Transcriptome analysis of avian reovirus-mediated changes in gene expression of normal chicken fibroblast DF-1 cells
【24h】

Transcriptome analysis of avian reovirus-mediated changes in gene expression of normal chicken fibroblast DF-1 cells

机译：禽呼肠孤病毒介导的正常鸡成纤维细胞DF-1细胞基因表达变化的转录组分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Avian reovirus (ARV) is an important poultry pathogen that can cause immunosuppression. In this study, RNA-Seq technology was applied to investigate the transcriptome-wide changes of DF-1 cells upon ARV infection at the middle stage. Total RNA of ARV-infected or mock-infected samples at 10 and 18?h post infection (hpi) was extracted to build RNA-Seq datasets. Analysis of the sequencing data revealed that the expressions of numerous genes were altered, and a panel of differentially expressed genes were confirmed with RT-qPCR. At 10 hpi, 104 genes were down-regulated and 64 were up-regulated, while the expressions of 47 genes were increased and only one was down-regulated, which may play a role in retinoic acid biosynthesis, at 18 hpi in the ARV-infected cells. The similar profiles of up-regulated genes between the two groups of infected cells suggest that ARV infection activated a prolonged antiviral response of host cells. Alternative splicing analysis found no significantly changed events altered by ARV infection. Overall, the differential expression profile presented in this study can be used to expand our understanding of the comprehensive interactions between ARV and the host cells, and may be helpful for us to reveal the pathogenic mechanism on the molecular level.
机译：禽呼肠孤病毒（ARV）是一种重要的禽类病原体，可引起免疫抑制。在这项研究中，RNA-Seq技术用于研究在中期ARV感染后DF-1细胞在转录组范围内的变化。提取感染后10和18？h（hpi）的ARV感染或模拟感染样品的总RNA，以构建RNA-Seq数据集。对测序数据的分析表明，许多基因的表达均发生了改变，并且通过RT-qPCR证实了一组差异表达的基因。在10 hpi时，ARV-的18 hpi下调了104个基因，上调了64个基因，而47个基因的表达增加了，而只有一个下调，这可能在视黄酸的生物合成中起作用。被感染的细胞。两组感染细胞之间的上调基因的相似图谱表明，ARV感染激活了宿主细胞延长的抗病毒应答。替代剪接分析未发现由ARV感染改变的明显变化的事件。总体而言，本研究中呈现的差异表达谱可用于扩展我们对ARV与宿主细胞之间全面相互作用的理解，并可能有助于我们在分子水平上揭示其致病机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号