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首页> 外文期刊>BMC Genomics >Identification and characterization of miRNAs in the gills of the mud crab ( Scylla paramamosain ) in response to a sudden drop in salinity
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Identification and characterization of miRNAs in the gills of the mud crab ( Scylla paramamosain ) in response to a sudden drop in salinity

机译：识别和表征泥蟹the（Sylla paramamosain）中的miRNA，以应对盐度的突然下降

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摘要

The mud crab (Scylla paramamosain) is a euryhaline and commercially important species. MiRNAs participate in the regulation of many physiological activities. The miRNA transcriptome of the gills of S. paramamosain was used to investigate the expression profiles of miRNAs in response to a sudden drop in salinity. In total, seven known miRNAs and 43 novel miRNAs were identified, with 18 differentially expressed small RNAs. Fourteen thousand nine hundred fifty-one differentially expressed miRNAs target genes were screened by prediction. GO analysis of differentially expressed miRNAs target genes indicated that 578 genes associated with cellular processes, 523 associated with metabolic processes, and 422 associated with single-organism processes were the most strongly affected by a sudden drop in salinity from 23‰ to 3‰. KEGG pathway analysis showed 14 pathways were related to amino acid metabolism, which plays an important role in osmoregulation. Besides, several pathways were associated with starch and sucrose metabolism (ko00500), glycosaminoglycan degradation (ko00531), and galactose metabolism (ko00052). S. paramamosain regulated osmotic pressure and energy balance by regulating target genes to adapt to a sudden changes in salinity. These results provided a basis for further investigations of miRNA-modulating networks underlying the osmoregulation of S. paramamosain.
机译：泥蟹（Scylla paramamosain）是一种淡碱性，在商业上很重要。 MiRNA参与许多生理活动的调节。副产沙门氏菌腮的miRNA转录组用于研究盐度突然下降时miRNA的表达情况。总共鉴定出七个已知的miRNA和43个新颖的miRNA，以及18个差异表达的小RNA。通过预测筛选了1 491个差异表达的miRNA目标基因。差异表达的miRNA靶基因的GO分析表明，盐度从23‰降至3‰时，与细胞过程相关的578个基因，与代谢过程相关的523个基因和与单一生物过程相关的422个基因受到的影响最大。 KEGG通路分析显示14种通路与氨基酸代谢有关，在渗透调节中起着重要作用。此外，几种途径与淀粉和蔗糖代谢（ko00500），糖胺聚糖降解（ko00531）和半乳糖代谢（ko00052）有关。 S. paramamosain通过调节靶基因来适应盐度的突然变化，从而调节渗透压和能量平衡。这些结果为进一步研究副果糖链霉菌渗透调节基础的miRNA调节网络提供了基础。

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