...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Genome-wide identification and comprehensive analysis of microRNAs and phased small interfering RNAs in watermelon
【24h】

Genome-wide identification and comprehensive analysis of microRNAs and phased small interfering RNAs in watermelon

机译：西瓜中微小RNA和分阶段的小干扰RNA的全基因组鉴定和综合分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs involved in the post-transcriptional gene regulation and play a critical role in plant growth, development and stress responses. Watermelon (Citrullus lanatus L.) is one of the important agricultural crops worldwide. However, the watermelon miRNAs and phasiRNAs and their functions are not well explored. Here we carried out computational and experimental analysis of miRNAs and phased small interfering RNAs (phasiRNAs) in watermelon by analyzing 14 small RNA profiles from roots, leaves, androecium, petals, and fruits, and one published small RNA profile of mixed tissues. To identify the targets of miRNAs and phasiRNAs, we generated a degradome profile for watermelon leaf which is analyzed using the SeqTar algorithm. We identified 97 conserved pre-miRNAs, of which 58 have not been reported previously and 348 conserved mature miRNAs without precursors. We also found 9 novel pre-miRNAs encoding 18 mature miRNAs. One hundred and one 21 nucleotide (nt) PHAS loci, and two hundred and forty one 24 nt PHAS loci were also identified. We identified 127 conserved targets of the conserved miRNAs and TAS3-derived tasiRNAs by analyzing a degradome profile of watermelon leaf. The presented results provide a comprehensive view of small regulatory RNAs and their targets in watermelon.
机译：微小RNA（miRNA）是一类内源性小非编码RNA，参与转录后基因调控，并在植物生长，发育和胁迫反应中发挥关键作用。西瓜（Citrullus lanatus L.）是全世界重要的农作物之一。然而，西瓜miRNA和phasiRNA及其功能尚未得到很好的探索。在这里，我们通过分析来自根，叶，雄蕊，花瓣和果实的14种小RNA图谱以及一份已发表的混合组织小RNA图谱，对西瓜中的miRNA和分阶段的小干扰RNA（phasiRNAs）进行了计算和实验分析。为了确定miRNA和phasiRNA的靶标，我们生成了西瓜叶片的降解组图谱，并使用SeqTar算法对其进行了分析。我们鉴定了97个保守的pre-miRNA，其中58个以前没有报道过，还有348个没有前体的成熟miRNA。我们还发现了9种新颖的pre-miRNA，它们编码18种成熟的miRNA。还鉴定了101个21核苷酸（nt）的PHAS基因座和241个24nt的PHAS基因座。我们通过分析西瓜叶片的降解组图谱，鉴定了127个保守的miRNA和TAS3衍生的tasiRNA的保守靶标。呈现的结果提供了西瓜中小调节RNA及其靶标的全面视图。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号