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首页> 外文期刊>BMC Genomics >Characterization of the equine skeletal muscle transcriptome identifies novel functional responses to exercise training
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Characterization of the equine skeletal muscle transcriptome identifies novel functional responses to exercise training

机译：马骨骼肌转录组的表征鉴定了运动训练的新功能反应

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Background Digital gene expression profiling was used to characterize the assembly of genes expressed in equine skeletal muscle and to identify the subset of genes that were differentially expressed following a ten-month period of exercise training. The study cohort comprised seven Thoroughbred racehorses from a single training yard. Skeletal muscle biopsies were collected at rest from the gluteus medius at two time points: T1 - untrained, (9 ± 0.5 months old) and T2 - trained (20 ± 0.7 months old). Results The most abundant mRNA transcripts in the muscle transcriptome were those involved in muscle contraction, aerobic respiration and mitochondrial function. A previously unreported over-representation of genes related to RNA processing, the stress response and proteolysis was observed. Following training 92 tags were differentially expressed of which 74 were annotated. Sixteen genes showed increased expression, including the mitochondrial genes ACADVL, MRPS21 and SLC25A29 encoded by the nuclear genome. Among the 58 genes with decreased expression, MSTN, a negative regulator of muscle growth, had the greatest decrease. Functional analysis of all expressed genes using FatiScan revealed an asymmetric distribution of 482 Gene Ontology (GO) groups and 18 KEGG pathways. Functional groups displaying highly significant (P Conclusion Exercise training in Thoroughbred racehorses results in coordinate changes in the gene expression of functional groups of genes related to metabolism, oxidative phosphorylation and muscle structure.
机译：背景技术数字基因表达谱用于表征马骨骼肌中表达的基因的装配，并鉴定经过十个月的运动训练后差异表达的基因的子集。该研究队列包括来自单个训练场的七匹纯种赛马。静息时在两个时间点从臀中肌采集骨骼肌活检：T 1 -未经训练（9±0.5个月大）和T 2 -训练（20± 0.7个月大）。结果肌肉转录组中最丰富的mRNA转录物是那些参与肌肉收缩，有氧呼吸和线粒体功能的转录物。观察到与RNA加工，应激反应和蛋白水解有关的基因的先前未报道的过度表达。训练后，差异表达了92个标签，其中有74个被注释。十六个基因显示出增加的表达，包括核基因组编码的线粒体基因ACADVL，MRPS21和SLC25A29。在表达下降的58个基因中，MSTN是肌肉生长的负调节剂，下降幅度最大。使用FatiScan对所有表达的基因进行功能分析，发现482个基因本体（GO）组和18条KEGG途径的不对称分布。官能团表现出很高的意义（P结论在纯种赛马中进行运动训练会导致与代谢，氧化磷酸化和肌肉结构相关的基因的功能组的基因表达发生协调变化。

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