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首页> 外文期刊>BMC Genomics >Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation
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Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation

机译：哺乳期和非哺乳期牛乳腺中的microRNA的表达谱和与泌乳有关的miRNA的鉴定

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Background MicroRNAs (miRNAs) have been implicated in the regulation of milk protein synthesis and development of the mammary gland (MG). However, the specific functions of miRNAs in these regulations are not clear. Therefore, the elucidation of miRNA expression profiles in the MG is an important step towards understanding the mechanisms of lactogenesis. Results Two miRNA libraries were constructed from MG tissues taken from a lactating and a non-lactating Holstein dairy cow, respectively, and the short RNA sequences (18–30 nt) in these libraries were sequenced by Solexa sequencing method. The libraries included 885 pre-miRNAs encoding for 921 miRNAs, of which 884 miRNAs were unique sequences and 544 (61.5%) were expressed in both periods. A custom-designed microarray assay was then performed to compare miRNA expression patterns in the MG of lactating and non-lactating dairy cows. A total of 56 miRNAs in the lactating MG showed significant differences in expression compared to non-lactating MG (P Conclusion Our study provides a broad view of the bovine MG miRNA expression profile characteristics. Eight hundred and eighty-four miRNAs were identified in bovine MG. Differences in types and expression levels of miRNAs were observed between lactating and non-lactating bovine MG. Systematic predictions aided in the identification of lactation-related miRNAs, providing insight into the types of miRNAs and their possible mechanisms in regulating lactation.
机译：背景技术MicroRNA（miRNA）已参与乳蛋白合成和乳腺（MG）发育的调控。但是，miRNA在这些法规中的具体功能尚不清楚。因此，阐明MG中的miRNA表达谱是了解泌乳发生机理的重要一步。结果分别从哺乳期和非哺乳期荷斯坦奶牛的MG组织构建了两个miRNA文库，并通过Solexa测序方法对这些文库中的短RNA序列（18-30 nt）进行了测序。该文库包含885个pre-miRNA，编码921个miRNA，其中884个miRNA是唯一序列，在两个时期均表达544个（61.5％）。然后进行了定制设计的微阵列分析，以比较泌乳和非泌乳奶牛的MG中的miRNA表达模式。与非哺乳期的MG相比，泌乳期MG中共有56个miRNA在表达上有显着差异（P结论我们的研究为牛MG的miRNA表达谱特征提供了广阔的视野。在牛MG中鉴定出884个miRNA。在哺乳期和非哺乳期牛MG之间观察到miRNA的类型和表达水平的差异，系统的预测有助于鉴定与泌乳有关的miRNA，从而深入了解miRNA的类型及其调控泌乳的可能机制。

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