...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Quantitative phosphoproteomic profiling of fiber differentiation and initiation in a fiberless mutant of cotton
【24h】

Quantitative phosphoproteomic profiling of fiber differentiation and initiation in a fiberless mutant of cotton

机译：棉花无纤维突变体中纤维分化和启动的磷酸化蛋白质组学定量分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The cotton (Gossypium spp.) fiber cell is an important unicellular model for studying cell differentiation. There is evidence suggesting that phosphorylation is a critical post-translational modification involved in regulation of a wide range of cell activities. Nevertheless, the sites of phosphorylation in G. hirsutum and their regulatory roles in fiber cell initiation are largely unknown. In this study, we employed a mass spectrometry-based phosphoproteomics to conduct a global and site-specific phosphoproteome profiling between ovules of a fuzzless-lintless (fl) Upland cotton (G. hirsutum) mutant and its isogenic parental wild type (WT) at -3 and 0?days post-anthesis (DPA). Results A total of 830 phosphopeptides and 1,592 phosphorylation sites from 619 phosphoproteins were identified by iTRAQ (isobaric tags for relative and absolute quantitation). Of these, 76 phosphoproteins and 1,100 phosphorylation sites were identified for the first time after searching the P3DB public database using the BLAST program. Among the detected phosphopeptides, 69 were differentially expressed between the fl mutant and its WT in ovules at -3 and 0 DPA. An analysis using the Motif-X program uncovered 19 phosphorylation motifs, 8 of which were unique to cotton. A further metabolic pathway analysis revealed that the differentially phosphorylated proteins were involved in signal transduction, protein modification, carbohydrate metabolic processes, and cell cycle and cell proliferation. Conclusions Our phosphoproteomics-based research provides the first global overview of phosphorylation during cotton fiber initiation, and also offers a helpful dataset for elucidation of signaling networks in fiber development of G. hirsutum.
机译：背景技术棉（棉花属）纤维细胞是研究细胞分化的重要单细胞模型。有证据表明，磷酸化是重要的翻译后修饰，参与多种细胞活性的调节。然而，在陆地棉中的磷酸化位点及其在纤维细胞起始中的调控作用尚不清楚。在这项研究中，我们采用了基于质谱的磷酸化蛋白质组学，以在无绒棉（fl）陆地棉（G. hirsutum）突变体的胚珠与其同基因的亲本野生型（WT）之间进行全局和位点特定的磷酸化蛋白质组分析。花后-3天和0天（DPA）。结果通过iTRAQ（等压相对和绝对定量标记）共鉴定了619个磷蛋白中的830个磷酸肽和1,592个磷酸化位点。其中，使用BLAST程序搜索P 3 DB公共数据库后，首次鉴定出76个磷蛋白和1,100个磷酸化位点。在检测到的磷酸肽中，在-3和0 DPA的胚珠中，f1突变体与其WT之间的差异表达有69种。使用Motif-X程序进行的分析发现了19个磷酸化基序，其中8个是棉花特有的。进一步的代谢途径分析表明，差异磷酸化的蛋白质与信号转导，蛋白质修饰，碳水化合物代谢过程以及细胞周期和细胞增殖有关。结论我们基于磷酸化蛋白质组学的研究为棉花纤维起始过程中的磷酸化提供了第一份全球综述，也为阐明陆地棉纤维发育中的信号网络提供了有用的数据集。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号