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首页> 外文期刊>BMC Genomics >Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish
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Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish

机译：基因型和温度依赖性性别决定雄性之间不同的精子核行为与雌生殖鱼类中复制和表达相关的途径有关

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Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue. We found out that males of GSD and TSD in gibel carp had similar morphology, testicular histology, sperm structure and sperm vitality. However, when maternal individuals were mated with males of GSD, sperm nucleus swelling and fusing with the female pronucleus were observed in the fertilized eggs. On the contrary, when maternal individuals were mated with males of TSD, sperm nucleus remained in the condensed status throughout the whole process. Subsequently, semen proteomics analysis unveiled that DNA replication and gene expression-related pathways were inhibited in the sperm from males of TSD compared to males of GSD, and most differentially expressed proteins associated with DNA replication, transcription and translation were down-regulated. Moreover, via BrdU incorporation and immunofluorescence detection, male nucleus replication was revealed to be present in the fertilized eggs by the sperm from males of GSD, but absent in the fertilized eggs by the sperm from males of TSD. These findings indicate that DNA replication and gene expression-related pathways are associated with the distinct sperm nucleus development behaviors in fertilized eggs in response to the sperm from males of GSD and TSD. And this study is the first attempt to screen the differences between males determined via GSD and TSD in gynogenetic species, which might give a hint for understanding evolutionary adaption of diverse sex determination mechanisms in unisexual vertebrates.
机译：在一些吸热物种中已经揭示了多种性别决定策略的共存和过渡，但是由不同性别决定策略引起的男性间差异及其潜在机制仍不清楚。在这里，我们使用了遗传雌性长鳍鲤（Carassius gibelio）进行基因型性别确定（GSD）和温度依赖性性别确定（TSD）策略，以说明这一问题。我们发现，长鳍金枪鱼的GSD和TSD雄性具有相似的形态，睾丸组织学，精子结构和精子活力。然而，当产妇个体与GSD雄性交配时，在受精卵中观察到精子核膨胀和与雌性原核融合。相反，当产妇个体与TSD男性交配时，精子核在整个过程中仍处于浓缩状态。随后，精液蛋白质组学分析显示，与GSD雄性相比，TSD雄性精子中的DNA复制和基因表达相关途径受到抑制，并且与DNA复制，转录和翻译相关的大多数差异表达蛋白被下调。此外，通过BrdU掺入和免疫荧光检测，发现GSD雄性的精子在受精卵中存在雄核复制，而TSD雄性的精子在受精卵中不存在雄核复制。这些发现表明，DNA复制和基因表达相关的途径与受精卵对GSD和TSD雄性精子的反应不同，它们的精子核发育行为不同。这项研究是首次尝试筛选通过GSD和TSD确定的雌雄同体物种中的雄性差异，这可能为理解单性脊椎动物中各种性别决定机制的进化适应性提供提示。

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