• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo
【24h】

Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

机译：在整个动情周期中解读输卵管细胞外囊泡的含量：对配子-输卵管相互作用和潜在胚胎环境的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The success of early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players in mediating this crucial cross-talk by transferring their cargo (proteins, mRNA and small ncRNA) from cell to cell. However, little is known about the oviductal EVs (oEVS) composition and their implications in the reproductive success. The aim of the study was to determine the oEVs content at protein, mRNA and small RNA level and to examine whether the oEVs content is under the hormonal influence of the estrous cycle. We identified the presence of oEVs, exosomes and microvesicles, in the bovine oviductal fluid at different stages of the estrous cycle (postovulatory-stage, early luteal phase, late luteal phase and pre-ovulatory stage) and demonstrated that their composition is under hormonal regulation. RNA-sequencing identified 903 differentially expressed transcripts (FDR 2). Our data revealed proteins related to early embryo development and gamete-oviduct interactions as well as numerous ribosomal proteins. Our study provides with the first molecular signature of oEVs across the bovine estrous cycle, revealing marked differences between post- and pre-ovulatory stages. Our findings contribute to a better understanding of the potential role of oEVs as modulators of gamete/embryo-maternal interactions and their implications for the reproductive success.
机译：早期生殖活动的成功取决于配子/胚胎与输卵管之间的适当沟通。输卵管分泌物中所含的细胞外囊泡（EVs）已被认为是通过将它们的货物（蛋白质，mRNA和小ncRNA）从一个细胞转移到另一个细胞来介导这种重要串扰的新参与者。然而，关于输卵管电动车（oEVS）的组成及其对生殖成功的影响知之甚少。这项研究的目的是确定蛋白质，mRNA和小RNA水平上的oEV含量，并检查oEV含量是否受发情周期的激素影响。我们确定了在动情周期的不同阶段（排卵后阶段，黄体早期，黄体晚期和排卵前阶段）在牛输卵管中存在oEV，外泌体和微泡，并证明它们的组成受激素调节。 。 RNA测序鉴定了903个差异表达的转录本（FDR 2）。我们的数据揭示了与早期胚胎发育和配子输卵管相互作用有关的蛋白质，以及许多核糖体蛋白质。我们的研究提供了整个牛动情周期中oEV的第一个分子标记，揭示了排卵后和排卵前阶段之间的显着差异。我们的发现有助于更好地理解oEV作为配子/胚胎-母体相互作用调节剂的潜在作用及其对生殖成功的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号