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首页> 外文期刊>BMC Genomics >A high density SLAF-SNP genetic map and QTL detection for fibre quality traits in Gossypium hirsutum
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A high density SLAF-SNP genetic map and QTL detection for fibre quality traits in Gossypium hirsutum

机译：陆地棉纤维品质性状的高密度SLAF-SNP遗传图谱和QTL检测

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Upland Cotton (Gossypium hirsutum) is a very important cash crop known for its high quality natural fiber. Recent advances in sequencing technologies provide powerful tools with which to explore the cotton genome for single nucleotide polymorphism marker identification and high density genetic map construction toward more reliable quantitative trait locus mapping. In the present study, a RIL population was developed by crossing a Chinese high fiber quality cultivar (Yumian 1) and an American high fiber quality line (CA3084), with distinct genetic backgrounds. Specific locus amplified fragment sequencing (SLAF-seq) technology was used to discover SNPs, and a genetic map containing 6254 SNPs was constructed, covering 3141.72 cM with an average distance of 0.5 cM between markers. A total of 95 QTL were detected for fiber quality traits in three environments, explaining 5.5-24.6% of the phenotypic variance. Fifty-five QTL found in multiple environments were considered stable QTL. Nine of the stable QTL were found in all three environments. We identified 14 QTL clusters on 13 chromosomes, each containing one or more stable QTL. A high-density genetic map of Gossypium hirsutum developed by using specific locus amplified fragment sequencing technology provides detailed mapping of fiber quality QTL, and identification of ‘stable QTL’ found in multiple environments. A marker-rich genetic map provides a foundation for fine mapping, candidate gene identification and marker-assisted selection of favorable alleles at stable QTL in breeding programs.
机译：陆地棉（陆地棉）是一种非常重要的经济作物，以优质天然纤维闻名。测序技术的最新进展提供了强大的工具，可用于探索棉花基因组以进行单核苷酸多态性标记鉴定和高密度遗传图谱构建，以实现更可靠的定量性状基因座图谱。在本研究中，通过杂交具有不同遗传背景的中国高纤维品质品种（榆面1）和美国高纤维品质品系（CA3084）开发了RIL种群。使用特异性基因座扩增片段测序（SLAF-seq）技术发现SNP，并构建了包含6254个SNP的遗传图谱，覆盖了3141.72 cM，标记之间的平均距离为0.5 cM。在三个环境中共检测到95个QTL的纤维品质性状，解释了5.5-24.6％的表型变异。在多个环境中发现的55个QTL被认为是稳定的QTL。在这三个环境中均发现了九个稳定的QTL。我们在13条染色体上鉴定了14个QTL簇，每个簇包含一个或多个稳定的QTL。通过使用特定的基因座扩增片段测序技术开发的陆地棉高密度遗传图谱，可以对纤维质量QTL进行详细的定位，并鉴定在多种环境中发现的“稳定QTL”。富含标记的遗传图谱为育种计划中稳定QTL的精细定位，候选基因鉴定和有利等位基因的标记辅助选择提供了基础。

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