Adriamycin (10 mg/kg) administered s.c. to male ACI rats bearing Hepatoma H-4-II-E caused a 9-day delay in tumour growth but no changes in the clonogenic fraction of the tumour were detectable by in vitro assay at any time after treatment. There is no significant decrease in the yield of cells on enzymatic dissociation of the tumour nor a reduction in mg DNA/g tumour that might indicate a decrease in tumour cellularity. Mitotic and labelling indices and [3H]dT uptake into DNA remain essentially unchanged, relative to age-equivalent controls, but are slightly lower than in controls of equal weight. The reliability of the clonogenic assay and possible mechanisms by which Adriamycin delays tumour growth are discussed.
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机译：皮下注射阿霉素（10 mg / kg）对患有肝癌H-4-II-E的雄性ACI大鼠的抗药性导致肿瘤生长延迟了9天，但在治疗后的任何时间都无法通过体外测定法检测到肿瘤的克隆形成部分的变化。肿瘤的酶解离后细胞产量没有显着降低，mg DNA / g肿瘤的降低也没有表明肿瘤细胞的减少。相对于年龄相同的对照组，有丝分裂和标记指数以及[3H] dT对DNA的吸收基本保持不变，但比同等重量的对照组略低。讨论了克隆形成试验的可靠性以及阿霉素延缓肿瘤生长的可能机制。
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