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首页> 外文期刊>British Journal of Cancer >Immunological Studies in Acute Myeloid Leukaemia: PHA Responsiveness and Serum Inhibitory Factors
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Immunological Studies in Acute Myeloid Leukaemia: PHA Responsiveness and Serum Inhibitory Factors

机译：急性骨髓性白血病的免疫学研究：PHA反应性和血清抑制因子。

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Sera from 16 of 20 patients with AML at some stage of the disease inhibited the in vitro PHA transformation of normal lymphocytes assessed by measuring the rate of DNA synthesis after 67-70 hours; 42% of pretreatment sera were inhibitory. Inhibitory activity was overcome at PHA concentrations 2-3 times greater than the concentration which allowed maximum discrimination between NHS and leukaemia sera.PHA transformation of washed lymphocytes obtained from AML patients before treatment and when receiving induction or consolidation (cytoreductive) chemotherapy was reduced only when cultures contained a high proportion of primitive cells. Even in primitive cell contaminated cultures significant responses to PHA could be measured if conditions were modified to prevent increasing acidity.Reports of reduced in vitro immunological reactions in pretreatment and poor prognosis patients may therefore be due to the presence of primitive cells in culture, and in treated patients to the failure of chemotherapy to reduce the circulating primitive cell count. Serum inhibitory factors may have a significant immunosuppressive effect in vivo, but the accurate assessment of the role of immune mechanisms in AML should attempt the measurement of specific immunity.
机译：通过测量67-70小时后的DNA合成速率来评估，在该疾病某个阶段的20例AML患者中，有16例的血清抑制了正常淋巴细胞的体外PHA转化;预处理血清中有42％具有抑制作用。 PHA浓度比允许最大区分NHS和白血病血清的PHA浓度高2-3倍时克服了抑制活性。从AML患者治疗前和接受诱导或巩固（细胞还原）化学疗法后洗出的淋巴细胞的PHA转化仅在以下情况下降低：培养物中含有大量原始细胞。甚至在原始细胞污染的培养物中，如果通过修改条件以防止酸度升高也可以测量出对PHA的显着反应。因此，有报道称在预处理和预后不良的患者中体外免疫学反应减少了，这可能是由于培养物中存在原始细胞所致。治疗的患者因化疗失败而减少了循环中的原始细胞数量。血清抑制因子可能在体内具有明显的免疫抑制作用，但准确评估AML中免疫机制的作用应尝试测量特异性免疫。

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