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首页> 外文期刊>British Journal of Cancer >LRIG1 and epidermal growth factor receptor in renal cell carcinoma: a quantitative RT|[ndash]|PCR and immunohistochemical analysis
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LRIG1 and epidermal growth factor receptor in renal cell carcinoma: a quantitative RT|[ndash]|PCR and immunohistochemical analysis

机译：肾细胞癌中LRIG1和表皮生长因子受体的定量RT | ndashPCR和免疫组化分析

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摘要

In all, 31 renal cell carcinomas (RCCs) were examined for expression of the potential tumour suppressor LRIG1 (formerly Lig-1) and the epidermal growth factor receptor (EGFR). Eight matched samples of uninvolved kidney cortex were also evaluated. Gene expression was examined by quantitative real-time RT–PCR. In the eight matched sample pairs (uninvolved kidney cortex and tumour), protein expression was examined by immunohistochemistry. Conventional (clear cell) tumours showed an expected upregulation of EGFR. LRIG1 expression was generally downregulated in conventional and papillary RCC but not in chromophobic RCC. The ratio between EGFR and LRIG1 was more than 2.5-fold higher in the eight tumours compared with matched uninvolved kidney cortex and was at least two-fold higher than the mean normal ratio in 21 of 31 samples analysed. The observed downregulation of LRIG1 and increased EGFR/LRIG1 ratios are consistent with LRIG1 being a suppressor of oncogenesis in RCC by counteracting the tumour-promoting properties of EGFR. Further studies are justified to elucidate the explicit role of LRIG1 in the oncogenesis of RCC.
机译：总共检查了31个肾细胞癌（RCC）的潜在肿瘤抑制因子LRIG1（以前称为Lig-1）和表皮生长因子受体（EGFR）的表达。还评估了八个匹配的未受累肾皮质样品。通过定量实时RT-PCR检查基因表达。在八个匹配的样本对（未涉及的肾皮质和肿瘤）中，通过免疫组织化学检查了蛋白质表达。常规（透明细胞）肿瘤显示出预期的EGFR上调。在常规和乳头状RCC中，LRIG1表达通常被下调，而在发色RCC中则没有。与匹配的未受累的肾皮质相比，在八个肿瘤中，EGFR和LRIG1的比率高出2.5倍以上，并且比分析的31个样本中的21个平均正常比率高至少两倍。观察到的LRIG1的下调和EGFR / LRIG1比率的增加与LRIG1通过抵消EGFR的促肿瘤特性而抑制RCC中的肿瘤发生是一致的。有理由进行进一步的研究以阐明LRIG1在RCC的肿瘤发生中的明确作用。

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