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首页> 外文期刊>British Journal of Cancer >Establishment and characterisation of cell lines from patients with lung cancer (predominantly small cell carcinoma)
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Establishment and characterisation of cell lines from patients with lung cancer (predominantly small cell carcinoma)

机译：肺癌（主要是小细胞癌）患者细胞系的建立和表征

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Tissue samples from 59 patients with lung cancer have been used to establish cell lines in culture. The primary diagnosis was small cell carcinoma in all except four. Most of the samples were of bone marrow but pleural effusions, lymph node biopsies and skin metastases were also included. The samples were usually split between HITES serum-free medium and HITES plus 2.5% foetal calf serum. A total of 19 cell lines were established and characterised. One line is large cell anaplastic lung carcinoma, four are B-lymphoblastoid and fourteen are small cell lung cancer. Considerable heterogeneity in gross morphology, neuroendocrine differentiation (by electron microscopy) and content of the enzyme L-dopa decarboxylase was seen. The use of HITES plus 2.5% foetal calf serum resulted in better establishment of cultures than did serum-free HITES.
机译：来自59名肺癌患者的组织样本已用于建立培养细胞系。最初的诊断是除四个以外的所有小细胞癌。大多数样品是骨髓，但也包括胸腔积液，淋巴结活检和皮肤转移。通常将样品分为HITES无血清培养基和HITES加2.5％胎牛血清。建立并表征了总共19个细胞系。一系为大细胞间变性肺癌，四系为B淋巴母细胞样癌，十四系为小细胞肺癌。在总体形态，神经内分泌分化（通过电子显微镜）和L-多巴脱羧酶的含量方面观察到明显的异质性。与不含血清的HITES相比，使用HITES加2.5％的胎牛血清可更好地培养。

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