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首页> 外文期刊>British Journal of Cancer >Modulation of placental alkaline phosphatase activity and cytokeratins in human HN-1 cells by butyrate, retinoic acid, catecholamines and histamine
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Modulation of placental alkaline phosphatase activity and cytokeratins in human HN-1 cells by butyrate, retinoic acid, catecholamines and histamine

机译：丁酸酯，视黄酸，儿茶酚胺和组胺对人HN-1细胞中胎盘碱性磷酸酶活性和细胞角蛋白的调节

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摘要

The effects of butyrate and retinoic acid in combination with catecholamines or histamine on the HN-1 human head and neck squamous carcinoma cell line were investigated analysing cell proliferation, placental alkaline phosphatase (PLAP) activity, and relative cytokeratin content. Butyrate inhibited cell proliferation in agar, whereas retinoic acid induced a small inhibitory effect. Butyrate enhanced PLAP activity in a time related manner in contrast to retinoic acid, which had no significant effect. However, retinoic acid inhibited the efficacy of butyrate to induce PLAP activity. A synergistic enhancement of PLAP activity was demonstrated after treatment of butyrate pretreated cells with catecholamines or histamine. The beta-adrenergic antagonist propranolol partly inhibited the aforementioned enhancement of PLAP activity, whereas the alpha-adrenergic antagonist phentolamine further enhanced PLAP activity. Indirect labeling of keratins with a polyclonal antibody showed that cytokeratin content was enhanced by butyrate but not by retinoic acid. Further analysis of cytokeratin content using four monoclonal antibodies showed that labeling of cytokeratins (5 + 8) was increased by butyrate. PLAP activity could be modulated by a concerted action of either butyrate plus retinoic acid or butyrate plus catecholamines or histamine, indicating a possible role for PLAP in tumour cell proliferation.
机译：通过分析细胞增殖，胎盘碱性磷酸酶（PLAP）活性和相对细胞角蛋白含量，研究了丁酸酯和视黄酸联合儿茶酚胺或组胺对HN-1人头颈部鳞状细胞癌细胞系的影响。丁酸盐抑制琼脂中的细胞增殖，而视黄酸则具有很小的抑制作用。与视黄酸相反，丁酸以时间相关的方式增强PLAP活性，而视黄酸没有明显的作用。但是，视黄酸抑制了丁酸酯诱导PLAP活性的功效。用儿茶酚胺或组胺处理丁酸盐预处理的细胞后，PPL活性得到协同增强。 β-肾上腺素拮抗剂普萘洛尔部分抑制了上述PLAP活性的增强，而α-肾上腺素拮抗剂酚妥拉明进一步增强了PLAP活性。用多克隆抗体间接标记角蛋白表明，丁酸可增加细胞角蛋白的含量，而视黄酸则不会。使用四种单克隆抗体对细胞角蛋白含量进行的进一步分析显示，丁酸酯会增加细胞角蛋白的标记（5 + 8）。 PLAP活性可通过丁酸酯加视黄酸或丁酸酯加儿茶酚胺或组胺的协同作用来调节，表明PLAP在肿瘤细胞增殖中的可能作用。

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