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首页> 外文期刊>British Journal of Cancer >Mutations of the |[beta]| - and |[gamma]| -catenin genes are uncommon in human lung, breast, kidney, cervical and ovarian carcinomas
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Mutations of the |[beta]| - and |[gamma]| -catenin genes are uncommon in human lung, breast, kidney, cervical and ovarian carcinomas

机译：|β|的突变-和|γ| -catenin基因在人类肺癌，乳腺癌，肾脏癌，宫颈癌和卵巢癌中不常见

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β-catenin forms complexes with Tcf and Lef-1 and functions as a transcriptional activator in the Wnt signalling pathway. Although recent investigations have been focused on the role of the adenomatous polyposis coli (APC)/ β-catenin/Tcf pathway in human tumorigenesis, there have been very few reports on mutations of the β-catenin gene in a variety of tumour types. Using PCR and single-strand conformational polymorphism analysis, we examined 93 lung, 9 breast, 6 kidney, 19 cervical and 7 ovarian carcinoma cell lines for mutations in exon 3 of the β-catenin gene. In addition, we tested these same samples for mutations in the NH2-terminal regulatory region of the γ-catenin gene. Mutational analysis for the entire coding region of β-catenin cDNA was also undertaken in 20 lung, 9 breast, 5 kidney and 6 cervical carcinoma cell lines. Deletion of most β-catenin coding exons was confirmed in line NCI-H28 (lung mesothelioma) and a silent mutation at codon 214 in exon 5 was found in HeLa (cervical adenocarcinoma). A missense mutation at codon 19 and a silent mutation at codon 28 in the NH2-terminal regulatory region of the γ-catenin gene were found in H1726 (squamous cell lung carcinoma) and H1048 (small cell lung carcinoma), respectively. Neither deletions nor mutations of these genes were detected in the other cell lines examined. These results suggest that β- and γ-catenins are infrequent mutational targets during development of human lung, breast, kidney, cervical and ovarian carcinomas. ? 2001 Cancer Research Campaign http://www.bjcancer.com
机译：β-catenin与Tcf和Lef-1形成复合物，并在Wnt信号通路中起转录激活剂的作用。尽管最近的研究集中在腺瘤性息肉病大肠杆菌（APC）/β-catenin/ Tcf途径在人类肿瘤发生中的作用，但很少有关于多种肿瘤类型中β-catenin基因突变的报道。使用PCR和单链构象多态性分析，我们检查了93肺，9乳腺癌，6肾脏，19宫颈癌和7卵巢癌细胞系中β-catenin基因外显子3的突变。此外，我们测试了这些相同的样本中γ-catenin基因的NH2末端调节区中的突变。还对20种肺，9种乳腺癌，5种肾脏和6种宫颈癌细胞系进行了β-catenincDNA整个编码区的突变分析。在NCI-H28（肺间皮瘤）品系中确认了大多数β-catenin编码外显子的缺失，并且在HeLa（宫颈腺癌）中发现了外显子5中第214位密码子的沉默突变。在H1726（鳞状细胞肺癌）和H1048（小细胞肺癌）中分别发现了γ-catenin基因NH2末端调节区中第19个密码子的错义突变和第28个密码子的沉默突变。在检查的其他细胞系中都未检测到这些基因的缺失或突变。这些结果表明，β-和γ-连环蛋白是人类肺癌，乳腺癌，肾癌，宫颈癌和卵巢癌发展过程中的罕见突变靶标。 ？ 2001年癌症研究运动http://www.bjcancer.com

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