Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

D M Easty; G C Easty

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Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

机译：细胞体外渗透正常组织能力的测量

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Organ cultures of chorioallantoic membranes of hen eggs have been used to establish a quantitative method of measuring the infiltrative ability of a variety of normal and tumour cells. Normal fibroblasts, mouse peritoneal cells and cells of low tumorigenicity infiltrated poorly and slowly whereas most tumours infiltrated rapidly. Some cells of the more invasive tumours achieved minimum rates of migration through the normal tissue of 2-3 μm/h. One tumour line which tended to form aggregates on the chorioallantoic membrane elicited a pronounced rejection response from the ectoderm of the membrane. Colcemid, which inhibits the formation of cell processes and the directional migration of cells, and dibutyryl cyclic AMP, which restores certain aspects of normal behaviour to tumour cells in vitro, had little effect on the invasion of the membrane by tumour cells.

机译：鸡蛋的绒毛膜尿囊膜的器官培养已被用于建立定量方法，以测量多种正常细胞和肿瘤细胞的浸润能力。正常的成纤维细胞，小鼠腹膜细胞和低致瘤性细胞浸润缓慢，而大多数肿瘤浸润迅速。更具侵入性的肿瘤的某些细胞在正常组织中的最低迁移速率为2-3μm/ h。趋于在绒膜尿囊膜上形成聚集体的一种肿瘤系引起了来自膜外胚层的明显排斥反应。抑制细胞过程形成和细胞定向迁移的Colcemid，以及在体外恢复肿瘤细胞正常行为某些方面的二丁酰环AMP，对肿瘤细胞对膜的侵袭几乎没有影响。

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《British Journal of Cancer》 |1974年第1期|共14页
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D M Easty; G C Easty;
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中图分类肿瘤学;
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Measurement of the Ability of Cells to Infiltrate Normal Tissues in vitro

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