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首页> 外文期刊>British Journal of Cancer >The generation of monoclonal antibodies against human pancreatic exocrine cancer: A study of six different immunisation regimes
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The generation of monoclonal antibodies against human pancreatic exocrine cancer: A study of six different immunisation regimes

机译：抗人胰腺外分泌癌单克隆抗体的产生：六种不同免疫方案的研究

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Six different immunisation regimes have been used to generate spleen cells with reactivity against human pancreatic exocrine cancer. Immunised spleen cells were fused with an NSO/1 myeloma line and supernatants from these hybridomas selectively screened for monoclonal antibodies which bound predominantly to a pancreatic cancer cell line (GER). The spleen cells from hairy litter mates immunised with pancreatic cancer xenograft homogenates and viable GER cells generated 13% of hybridoma supernatants which showed some selectivity for GER pancreatic cancer cells in a fixed cell ELISA assay. The other methods produced only 4% of hybrids with selectivity for GER cells. The antigen distribution on gluteraldehyde fixed cells was similar to that found for viable cell monolayers but many antigens were unstable on formalin fixation. Immunohistochemical staining of GER cells grown on glass slides showed a heterogeneity of antigen distribution with up to 70% of the cells exhibiting a vesicular pattern of staining. Fifty percent of the antibodies which bound to GER cells were also reactive against antigens in formalin-fixed paraffin-embedded tissue sections of the original GER tumour. Monoclonal antibody DD9E7 identified an antigen expressed on 12/14 pancreatic adenocarcinomas. The antibody showed strong staining of malignant luminal membranes and cytoplasm. The antigen was also present in normal salivary and sweat glands, and colon and breast carcinomas, but its tissue distribution was unlike that of CEA or EMA. The expression of this antigen in 12/14 of pancreatic carcinomas suggests that DD9E7 may be a useful reagent for pancreatic tumour detection.
机译：已经使用六种不同的免疫方案来产生对人胰腺外分泌癌具有反应性的脾细胞。将免疫的脾细胞与NSO / 1骨髓瘤细胞系融合，并从这些杂交瘤的上清液中选择性筛选主要结合胰腺癌细胞系（GER）的单克隆抗体。用胰腺癌异种移植匀浆免疫的毛茸茸伴侣的脾细胞和活的GER细胞产生了13％的杂交瘤上清液，在固定细胞ELISA分析中对GER胰腺癌细胞显示出一定的选择性。其他方法仅产生对GER细胞具有选择性的4％杂种。戊二醛固定细胞上的抗原分布与活细胞单层发现的相似，但许多抗原在福尔马林固定后不稳定。在载玻片上生长的GER细胞的免疫组织化学染色显示出抗原分布的异质性，多达70％的细胞表现出囊泡染色模式。与GER细胞结合的抗体中有百分之五十也对原始GER肿瘤的福尔马林固定石蜡包埋的组织切片中的抗原具有反应性。单克隆抗体DD9E7鉴定了在12/14胰腺腺癌中表达的抗原。该抗体显示出恶性腔膜和细胞质的强烈染色。该抗原也存在于正常唾液腺，汗腺，结肠癌和乳腺癌中，但其组织分布与CEA或EMA不同。该抗原在胰腺癌的12/14中的表达表明DD9E7可能是检测胰腺肿瘤的有用试剂。

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