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首页> 外文期刊>British Journal of Cancer >Marker genes for circulating tumour cells predict survival in metastasized breast cancer patients
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Marker genes for circulating tumour cells predict survival in metastasized breast cancer patients

机译：循环肿瘤细胞的标记基因可预测转移性乳腺癌患者的生存

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We investigated the prognostic significance of circulating breast cancer cells in peripheral blood detected by quantitative RT–PCR of marker genes in patients with advanced breast cancer. Blood samples from 94 breast cancer patients with metastatic disease (M1) were examined for circulating tumour cells by studying the mRNA expression of CK19, p1B, PS2 and EGP2 by real-time PCR. Using a score function, developed for predicting circulating tumour cells by quadratic discriminant analysis (QDA), the four expression levels were combined into a single discriminant value. Tumour cells were present in 24 out of 94 (31%) of the patients. In 77% (72 out of 94) of the patients distant metastatic disease was localised in the bone. In 36% (26 out of 72) of the patients with bone metastases at the time of blood sampling, a positive QDA for the four genes was found, in contrast to only 14% (three out of 22) without bone involvement. Overall survival rates by Kaplan–Meier revealed no prognostic effect for the presence of bone metastases (P=0.93). However, patients with a positive QDA value did have a progression-free survival at 1 year of 3% and overall survival at 2 years of 17%, against 22 and 36% for patients with a negative QDA value (P=0.015 and 0.0053, respectively). Breast cancer patients with metastatic disease have a significantly worse progression-free and overall survival when circulating tumour cells can be detected in their peripheral blood.
机译：我们研究了定量RT-PCR检测标记基因对晚期乳腺癌患者外周血中循环乳腺癌细胞的预后意义。通过实时PCR研究CK19，p1B，PS2和EGP2的mRNA表达，检查了94名转移性乳腺癌（M1）乳腺癌患者的血液样本中是否存在循环肿瘤细胞。使用为通过二次判别分析（QDA）预测循环肿瘤细胞而开发的评分功能，将四个表达水平合并为一个判别值。 94名患者中有24名（31％）存在肿瘤细胞。在77％（94名患者中的72％）患者中，远处转移性疾病位于骨中。在采血时有骨转移的患者中，有36％（72名患者中有26名）发现四个基因的QDA阳性，而没有骨参与的只有14％（22名中有3名）。 Kaplan–Meier的总生存率显示，对于骨转移的存在没有预后影响（P = 0.93）。但是，QDA值为正的患者在1年时的无进展生存期为3％，而2年的总生存率为17％，而QDA值为负的患者则为22％和36％（P = 0.015）。和0.0053）。当可以在其外周血中检测到循环中的肿瘤细胞时，患有转移性疾病的乳腺癌患者的无进展生存率和总体生存率将明显恶化。

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