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首页> 外文期刊>British Journal of Cancer >T-cell receptor gene expression in tumour-infiltrating lymphocytes and peripheral blood lymphocytes of patients with nasopharyngeal carcinoma
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T-cell receptor gene expression in tumour-infiltrating lymphocytes and peripheral blood lymphocytes of patients with nasopharyngeal carcinoma

机译：鼻咽癌患者肿瘤浸润淋巴细胞和外周血淋巴细胞中T细胞受体基因的表达

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The T-cell receptor (TCR) repertoire expression of tumour-infiltrating lymphocytes (TILs) from 19 nasopharyngeal carcinoma (NPC) biopsies was compared with those of lymphocytes from 18 control nasopharyngeal biopsies. mRNA was extracted from these lymphocytes and the cDNA transcribed. A panel of 18 V alpha- and 21 V beta-specific primers was used to detect the TCR gene use from cDNA. The use of V alpha and V beta genes was restricted in TILs compared with lymphocytes from biopsies. The frequencies of V alpha 2, V alpha 3, V alpha 9, V alpha 10, V alpha 11, V alpha 13, V alpha 14, V alpha 15, V beta 11, V beta 15 and V beta 20 were decreased and the frequencies of V alpha 10 [Pc = 0.04; relative risk (RR) = 0.05], V alpha 11 (Pc = 0.02; RR = 0.07), V alpha 13 (Pc = 0.002; RR = 0), V alpha 14 (Pc = 0.04; RR = 0.05), V beta 14 (Pc = 0.001; RR = 0.03) and V beta 20 (Pc = 0.001; RR = 0.03) remained significantly reduced after correction for the number of families typed. The frequency of V alpha 17 was higher in NPC biopsies than in NPC PBLs (P = 0.05), and the frequency of V beta 15 was lower in NPC biopsies than in NPC PBLs (P = 0.02). The frequencies of V alpha 17 and V alpha 18 in HLA-B46+ patients were significantly lower (P = 0.009; P = 0.044) than in B46+ controls. The results suggest that the restriction of TCR gene use in NPC patients may be important in NPC pathogenesis.
机译：比较了来自19例鼻咽癌（NPC）活检组织的肿瘤浸润淋巴细胞（TIL）的T细胞受体（TCR）集合表达与来自18例对照鼻咽活检组织的淋巴细胞的T细胞受体（TCR）全集表达。从这些淋巴细胞中提取mRNA，并转录cDNA。使用一组18 V alpha和21 V beta特异性引物来检测cDNA中TCR基因的使用。与活检的淋巴细胞相比，VIL和Vβ基因在TIL中的使用受到限制。降低了V alpha 2，V alpha 3，V alpha 9，V alpha 10，V alpha 11，V alpha 13，V alpha 14，V alpha 15，V beta 11，V beta 15和V beta 20的频率，并且V alpha 10的频率[Pc = 0.04;相对风险（RR）= 0.05]，V alpha 11（Pc = 0.02; RR = 0.07），V alpha 13（Pc = 0.002; RR = 0），V alpha 14（Pc = 0.04; RR = 0.05），V beta校正类型的家庭数目后，14（Pc = 0.001; RR = 0.03）和V beta 20（Pc = 0.001; RR = 0.03）仍显着降低。 NPC活检中V alpha 17的频率高于NPC PBL（P = 0.05），NPC活检中V beta 15的频率低于NPC PBL（P = 0.02）。 HLA-B46 +患者中V alpha 17和V alpha 18的频率显着低于B46 +对照（P = 0.009; P = 0.044）。结果表明，在NPC患者中限制TCR基因的使用可能在NPC发病机制中起重要作用。

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