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首页> 外文期刊>British Journal of Cancer >The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines
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The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines

机译：初始/残留DNA损伤的比率预测了7种子宫颈癌细胞系的固有放射敏感性

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The single-cell gel electrophoresis (comet) assay was used to measure radiation-produced DNA double-strand breaks (dsbs) in a series of seven cervical tumour cell lines (ME180, HT3, C33A, C41, SiHa, MS751 and CaSki). The proportion of DNA dsbs was measured immediately after radiation treatment (initial damage) and 16 h later after incubation at 37 degrees C (residual damage). Linear dose-response curves were seen for initial (slopes 0.23-0.66) and residual (slopes 0.16-0.87) DNA dsbs. Neither of the slopes of the linear regression analysis on the initial and on the residual DNA dsbs dose-response curves (range 0-80 Gy) correlated with SF2 (surviving fraction at 2 Gy) measured after high- (HDR) or low-dose-rate (LDR) irradiation. An association was evident between SF2 after HDR and LDR irradiation and the ratio of the absolute level of initial and residual damage after a single dose of 60 Gy. However, a significant correlation was found between HDR (r= -0.78, P = 0.04) and LDR (r = -0.86, P = 0.03) SF2 values and the ratio of the slopes of the initial and residual DNA dsbs dose-response curves (range 0.47-0.99), representing the fraction of DNA damage remaining. These results indicate that the neutral comet assay can be used to predict radiosensitivity of cervical tumour cell lines by assessing the ratio of initial and residual DNA dsbs.
机译：单细胞凝胶电泳（彗星）测定法用于测量一系列七个宫颈肿瘤细胞系（ME180，HT3，C33A，C41，SiHa，MS751和CaSki）中辐射产生的DNA双链断裂（dsbs）。 DNA dsbs的比例在放射治疗后（初始损伤）和在37摄氏度温育后16小时（残余损伤）立即进行测量。对于初始（斜率0.23-0.66）和残余（斜率0.16-0.87）DNA dsbs，观察到线性剂量-响应曲线。高剂量（HDR）或低剂量后测得的初始和残留DNA dsbs剂量反应曲线（0-80 Gy范围）的线性回归分析的斜率均与SF2（2 Gy的存活分数）相关。速率（LDR）辐射。 HDR和LDR照射后SF2与单次60 Gy剂量后初始损伤和残余损伤的绝对水平之比之间存在明显关联。但是，发现HDR（r = -0.78，P = 0.04）和LDR（r = -0.86，P = 0.03）SF2值与初始和残留DNA dsbs剂量反应曲线的斜率之比之间存在显着相关性。 （范围0.47-0.99），代表剩余的DNA损伤分数。这些结果表明，中性彗星试验可用于通过评估初始和残留DNA dsbs的比率来预测宫颈肿瘤细胞系的放射敏感性。

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