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首页> 外文期刊>British Journal of Cancer >Human Myeloma Marrow Cells in Immunologically Deficient Mice
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Human Myeloma Marrow Cells in Immunologically Deficient Mice

机译：免疫缺陷小鼠的人类骨髓瘤骨髓细胞

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Intact human bone marrow cells from 7 patients with myelomatosis were inoculated intravenously into adolescent CBA mice rendered immunologically deficient by thymectomy followed by total body irradiation (600 rad). Each inoculum of human myeloma marrow cells and subsequent passages of intact mouse marrow and spleen cells resulted in the presence of morphological changes in the marrow, spleen and peripheral blood of a proportion of these mice which were closely similar to those seen in the human donor. A substantial amount of human immunoglobulin (IgG and IgA) was detected in the sera of some of the mice showing morphological changes. Mice prepared identically but remaining uninoculated or receiving intact human bone marrow cells from 3 patients with no evidence of haematological malignancy showed none of these changes when examined after similar intervals.There are at least 3 possible explanations for these findings: in mice receiving human myeloma marrow cells they might be accounted for by the persistence and replication of these cells in an immunologically deficient host. In mice receiving a first, second or third passage of abnormal mouse marrow and spleen cells they might similarly be accounted for by the survival and multiplication of a stem cell secreting both mouse and human immunoglobulins. Alternatively, the mouse stem cells may in some way have been transformed following infection by a transmissible agent originally present in the myeloma donor marrow cells.
机译：将来自7名骨髓瘤病患者的完整人类骨髓细胞静脉内接种到通过胸腺切除术随后全身照射（600拉德）而变得免疫缺陷的青春期CBA小鼠中。人骨髓瘤骨髓细胞的每种接种物以及完整小鼠骨髓和脾细胞的随后传代导致一部分小鼠的骨髓，脾脏和外周血中形态学改变的存在，与人类供体中所观察到的极为相似。在一些显示形态变化的小鼠的血清中检测到大量的人免疫球蛋白（IgG和IgA）。用相同的方法制备小鼠，但仍未接种疫苗或接受了3名患者的完整人骨髓细胞，但没有血液系统恶性肿瘤的证据，经过相似的间隔检查后，这些变化均未发现。这些发现至少有3种可能的解释：在接受人骨髓瘤的小鼠中这些细胞可能是由于这些细胞在免疫缺陷宿主中的持续存在和复制造成的。在接受异常小鼠骨髓和脾细胞第一次，第二次或第三次传代的小鼠中，它们可能同样由分泌小鼠和人免疫球蛋白的干细胞的存活和繁殖来解释。备选地，小鼠干细胞可以在感染后被最初存在于骨髓瘤供体骨髓细胞中的可传播剂转化。

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