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首页> 外文期刊>British Journal of Cancer >Macrophage colony-stimulating factor is expressed by an ovarian carcinoma subline and stimulates the c-myc proto-oncogene
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Macrophage colony-stimulating factor is expressed by an ovarian carcinoma subline and stimulates the c-myc proto-oncogene

机译：巨噬细胞集落刺激因子由卵巢癌亚线表达并刺激c-myc原癌基因

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摘要

A small, fast-growing and non-differentiated clone (N.1) derived from the heterogeneous human epithelial ovarian carcinoma cell line HOC-7 produces an autocrine/paracrine factor that is secreted into the cell culture supernatant. This factor is capable of enhancing mRNA levels of the proliferation-related oncogene c-myc in the more differentiated clone D3 and in normal human fibroblasts MRC.5, but also in N.1 cells themselves. Supernatants enriched for this paracrine/autocrine factor also confer a mitogenic stimulus as measured by [3H]thymidine incorporation. Trypsin can neutralise the stimulating activity of the secreted factor as well as monoclonal antibodies directed against macrophage colony-stimulating factor (M-CSF). We show that M-CSF and also M-CSF receptor are expressed in N.1 cells and that recombinant M-CSF induces c-myc transcript levels in N.1 cells. This investigation raises the possibility that M-CSF might be an autocrine growth factor in non-differentiated ovarian carcinomas. Inappropriate cytokine production could create a tumour-promoting microenvironment in this cancer type.
机译：来自异质人上皮性卵巢癌细胞系HOC-7的小的，快速生长且未分化的克隆（N.1）产生分泌到细胞培养上清液中的自分泌/旁分泌因子。该因子能够提高分化程度更高的克隆D3和正常人成纤维细胞MRC.5中以及N.1细胞本身中与增殖有关的癌基因c-myc的mRNA水平。富含这种旁分泌/自分泌因子的上清液也可以通过[3 H]胸苷掺入来产生促有丝分裂刺激。胰蛋白酶可以中和分泌因子以及针对巨噬细胞集落刺激因子（M-CSF）的单克隆抗体的刺激活性。我们显示M-CSF以及M-CSF受体在N.1细胞中表达，并且重组M-CSF在N.1细胞中诱导c-myc转录水平。这项研究提出了M-CSF可能是未分化卵巢癌中自分泌生长因子的可能性。在这种癌症类型中，不适当的细胞因子生产可能会产生促进肿瘤的微环境。

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