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首页> 外文期刊>British Journal of Cancer >Response of remission lymphocytes to autochthonous leukaemic myeloblasts
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Response of remission lymphocytes to autochthonous leukaemic myeloblasts

机译：缓解淋巴细胞对自发性白血病成肌细胞的反应

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Thymidine incorporation in vitro by remission lymphocytes from a total of 6 patients with acute myeloid leukaemia (AML) was measured following stimulation by autochthonous and allogeneic AML blasts and cell lines. The early peak response to autochthonous blasts in 2 of these patients (48-72 h) is consistent with the concept of a population of lymphocytes pre-immunized to antigens carried by the blasts. Although stimulation in one patient was increased in the presence of more stimulating (S) blasts than responding (R) lymphocytes, positive responses in other tests were obtained at an S : R ratio of 1 : 1-5. When different methods of treatment of the stimulating autochthonous blasts were compared with untreated cells, mitomycin C gave the highest stimulation indices 2 out of 3 tests. Tissue culture medium in which autochthonous blasts had been incubated for 3-5 days failed to stimulate either remission lymphocytes alone, or combined cultures of lymphocytes with autochthonous or allogeneic blasts, suggesting that mitogenic factors released from autochthonous blasts are not responsible for lymphocyte stimulation. Treatment of autochthonous or allogeneic AML blasts with glycine-HC1(pH 3-0) to remove putative "blocking" factors failed to increase the stimulatory capacity of the leukaemic blasts.
机译：在自体和同种异体AML母细胞和细胞系刺激后，测量了总共6例急性髓样白血病（AML）患者的缓解淋巴细胞中的胸腺嘧啶核苷掺入。在这些患者中有2位（48-72小时）对自体胚细胞的早期高峰反应与预免疫由胚细胞携带的抗原的淋巴细胞群的概念一致。尽管在存在更多的刺激（S）胚泡而不是响应（R）淋巴细胞的情况下，一名患者的刺激有所增加，但在其他测试中，S：R比率为1：1至1-5则获得了阳性反应。当将刺激性自体胚细胞的不同处理方法与未处理的细胞进行比较时，丝裂霉素C给出了最高的刺激指数（3个测试中有2个）。在其中培养了3-5天内的自体胚细胞的组织培养基不能单独刺激缓解淋巴细胞，也不能刺激淋巴细胞与自体或同种异体胚细胞的混合培养，这表明自体胚细胞释放的促有丝分裂因子对淋巴细胞的刺激不起作用。用甘氨酸-HCl（pH 3-0）治疗本地或同种异体AML母细胞，以消除假定的“阻断”因子，未能增加白血病母细胞的刺激能力。

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