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首页> 外文期刊>British Journal of Cancer >Expression and activation of erbB-2 and epidermal growth factor receptor in lung adenocarcinomas
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Expression and activation of erbB-2 and epidermal growth factor receptor in lung adenocarcinomas

机译：erbB-2和表皮生长因子受体在肺腺癌中的表达和激活

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ErbB-2 and EGFR (epidermal growth factor receptor) are expressed in lung adenocarcinomas and associated with a poor prognosis. Immunocytochemical analysis revealed erbB-2 and EGFR coexperession as a characteristic feature of most lung adenocarcinomas, and at levels of receptor expression present in bronchial epithelial cells. In primary lung tumours and cell lines, erbB-2 detected using Western blot analysis demonstrated low-level phosphotyrosine staining of the 185 kDa band, as compared with breast cancer cell lines. A549 and A427 lung adenocarcinoma cells treated with neu differentiation factor (NDF) showed increased erbB-2 phosphotyrosine staining, but to a much lesser extent than breast cancer cells. The lung cells were examined for expression of the potential autocrine growth factors NDF and transforming growth factor alpha (TGF-alpha) by Northern blot analysis. Both NDF and TFG-alpha mRNA were abundantly expressed in the A549 cells. NDF mRNA was highest during active cell proliferation and decreased in confluent cells or after treatment with the growth-inhibitory steroid dexamethasone. Primary tumours and cell lines expressed EGFR, showing higher basal level phosphotyrosine staining than erbB-2. Treatment with NDF and EGF (epidermal growth factor) stimulated cell growth, and in A549 cells the presence of both factors provided an additive increase in cell growth. The growth stimulus that ligand-activated erbB-2 and EGFR provides to lung adenocarcinoma cells may establish a background of continued cell proliferation over which other critical transforming events may occur.
机译：ErbB-2和EGFR（表皮生长因子受体）在肺腺癌中表达且预后不良。免疫细胞化学分析显示erbB-2和EGFR共表达是大多数肺腺癌的特征，并且在支气管上皮细胞中存在受体表达水平。在原发性肺部肿瘤和细胞系中，与乳腺癌细胞系相比，使用蛋白质印迹分析检测到的erbB-2证实了185 kDa条带的磷酸酪氨酸水平较低。用神经分化因子（NDF）处理的A549和A427肺腺癌细胞显示出erbB-2磷酸酪氨酸染色增加，但程度远低于乳腺癌细胞。通过Northern印迹分析检查肺细胞中潜在的自分泌生长因子NDF和转化生长因子α（TGF-α）的表达。 NDF和TFG-αmRNA均在A549细胞中大量表达。 NDF mRNA在活性细胞增殖期间最高，而在融合细胞中或用抑制生长的类固醇地塞米松处理后降低。原发性肿瘤和细胞系表达EGFR，与erbB-2相比，显示较高的基础水平磷酸酪氨酸染色。用NDF和EGF（表皮生长因子）处理可刺激细胞生长，并且在A549细胞中，这两种因子的存在均可促进细胞生长。配体激活的erbB-2和EGFR对肺腺癌细胞提供的生长刺激可能建立了持续细胞增殖的背景，在此背景下可能发生其他关键转化事件。

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