...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Journal of Cancer >Expression of p53 in urothelial cell cultures from tumour-bearing and tumour-free patients
【24h】

Expression of p53 in urothelial cell cultures from tumour-bearing and tumour-free patients

机译：荷瘤和无瘤患者尿道上皮细胞培养物中p53的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An explant culture technique was used to culture normal urothelium from patients with muscle-invasive bladder cancer (transitional cell carcinoma, TCC) (n = 11) and from non-tumour-bearing patients (n = 60). Cell cultures were examined for expression of p53 using the monoclonal antibody p53-240. There was a statistically significant increase in p53 expression in normal urothelial cell cultures from patients with TCC (P 5% of cells expressing p53. p53 expression in this subgroup was found to correlate with cell proliferation in vitro (r2 = 0.766). None of these urothelial specimens was observed to express p53 when paraffin-embedded preparations were stained with p53-D07 antibody prior to culture. The rate of cellular proliferation in this subgroup did not differ from that of normal urothelium from TCC patients. Twenty-two paraffin-embedded, muscle-invasive TCC specimens were also evaluated for p53 expression using p53-D07. The expression of p53 in these tumours did not differ from that observed in normal urothelial cell cultures from patients with TCC (P = 0.26). This study identifies an overexpression of p53 in normal urothelial cells from patients with TCC and in proliferating cultures from a significant subgroup of patients without malignant disease. Increased p53 expression in normal cultured urothelial cells from patients with bladder cancer implies a global change in the mechanisms controlling urothelial cell division. This may represent an early step in the pathway to carcinogenesis.
机译：外植体培养技术用于培养患有肌肉浸润性膀胱癌（移行细胞癌，TCC）（n = 11）和非肿瘤患者（n = 60）的正常尿道上皮。使用单克隆抗体p53-240检查细胞培养物中p53的表达。 TCC患者正常尿道上皮细胞培养物中p53表达有统计学上的显着增加（表达p53的细胞中P 5 ％。该亚组中p53的表达与体外细胞增殖相关（r2 = 0.766）。在培养前用石蜡包埋的制剂用p53-D07抗体染色后，观察到这些尿道上皮样本表达p53，该亚组的细胞增殖速率与TCC患者的正常尿路上皮没有差异。 ，还使用p53-D07对肌肉侵袭性TCC标本中的p53表达进行了评估，p53在这些肿瘤中的表达与正常TCC患者尿道上皮细胞培养中观察到的无差异（P = 0.26）。 TCC患者正常尿道上皮细胞中p53的表达以及无恶性疾病的显着亚组患者增殖培养物中p53的表达来自膀胱癌患者的正常培养的尿道上皮细胞中的n暗示着控制尿道上皮细胞分裂的机制的整体变化。这可能代表了致癌途径中的早期步骤。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号