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首页> 外文期刊>British Journal of Cancer >Heterogeneity in the in vitro survival and proliferation of human seminoma cells
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Heterogeneity in the in vitro survival and proliferation of human seminoma cells

机译：人精原细胞体外存活和增殖中的异质性

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The in vitro culture conditions allowing survival and initial proliferation of murine primordial germ cells from 10.5 days post coitum embryos, which include the use of a murine embryonal fibroblast (STO) feeder, were applied to 21 human seminomas, composed of tumour cells which are considered as the malignant counterparts of human primordial germ cells. Cells from 18 seminomas attached poorly to STO, and only a few survived through day 10. In contrast, three seminomas showed a higher degree of attachment. Two of them showed initial proliferation and enhanced survival: 30 days for tumour SE1 and 25 days for tumour SE3. Tumour SE1 was more extensively studied, using the culture conditions allowing the derivation of pluripotent embryonic stem cells from 8.5 days post coitum murine primordial germ cells, which include the use of STO feeder, stem cell factor, leukaemia inhibitory factor and basic fibroblast growth factor. The presence of stem cell factor was necessary and sufficient for colonies of tumour cells to form during the first 3 days of culture. While the cell number decreased after day 3 in medium without fetal calf serum, it increased until day 9 in medium containing fetal calf serum. No reprogramming of SE1 cells to pluripotent stem cells was observed. Our data indicate that seminomas form a tumour population with a heterogeneous in vitro behaviour not equivalent to that of 8.5-10.5 days post coitum murine primordial germ cells.
机译：将体外培养条件允许21天后从鼠体成纤维细胞胚胎存活10.5天起，鼠源原始生殖细胞得以存活和初始增殖，其中包括使用鼠胚成纤维细胞（STO）饲养器，这些条件被认为是由肿瘤细胞组成的人类精原细胞作为人类原始生殖细胞的恶性对应物。来自18个精原细胞的细胞与STO的附着力很弱，只有少数存活至第10天。相比之下，三个精原细胞的附着率更高。其中两个显示出初始增殖并提高了存活率：肿瘤SE1为30天，肿瘤SE3为25天。对SE1肿瘤进行了更广泛的研究，使用的培养条件允许从COSUM小鼠原始原始生殖细胞后8.5天衍生多能胚胎干细胞，包括使用STO饲养细胞，干细胞因子，白血病抑制因子和碱性成纤维细胞生长因子。干细胞因子的存在对于在培养的前三天中形成肿瘤细胞集落是必要的和充分的。在无胎牛血清的培养基中，细胞数在第3天后减少，而在有胎牛血清的培养基中，细胞数一直增加到第9天。没有观察到SE1细胞重编程为多能干细胞。我们的数据表明，精原细胞瘤形成的肿瘤种群具有异质的体外行为，不等同于在结肠小鼠原始原始生殖细胞后的8.5-10.5天。

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