Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting

D J E Peeters; B De Laere; G G Van den Eynden; S J Van Laere; F Rothé; M Ignatiadis; A M Sieuwerts; D Lambrechts; A Rutten; P A van Dam; P Pauwels; M Peeters; P B Vermeulen; L Y Dirix

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Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting

机译：使用Dielectrophoretic细胞分选从CellSearch富集的血液样本中半自动分离单个或高度纯化的肿瘤细胞并进行分子表征

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Background:Molecular characterisation of single circulating tumour cells (CTCs) holds considerable promise for predictive biomarker assessment and to explore CTC heterogeneity. We evaluate a new method, the DEPArray system, that allows the dielectrophoretic manipulation and isolation of single and 100% purified groups of CTCs from pre-enriched blood samples and explore the feasibility of their molecular characterisation.

机译：背景：单个循环肿瘤细胞（CTC）的分子表征在预测生物标志物评估和探索CTC异质性方面具有广阔的前景。我们评估了一种新的方法DEPArray系统，该方法允许通过双电泳操作和从预富集血样中分离出单个和100％纯化的四氯化碳组，并探讨了其分子表征的可行性。

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《British Journal of Cancer》 |2013年第6期|共10页
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D J E Peeters; B De Laere; G G Van den Eynden; S J Van Laere; F Rothé; M Ignatiadis; A M Sieuwerts; D Lambrechts; A Rutten; P A van Dam; P Pauwels; M Peeters; P B Vermeulen; L Y Dirix;
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中图分类肿瘤学;
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1. Efficiency of whole genome amplification of single circulating tumor cells enriched by CellSearch and sorted by FACS [J] . Joost F Swennenhuis, Joke Reumers, Kim Thys, Genome Medicine . 2013,第11期

机译：通过CellSearch富集并通过FACS分选的单个循环肿瘤细胞的全基因组扩增效率
2. Label-free continuous lateral magneto-dielectrophoretic microseparators for highly efficient enrichment of circulating nucleated cells from peripheral blood [J] . Jinhee Jung, Su-Kil Seo, Young-Don Joo, Sensors and Actuators . 2011,第1期

机译：无标记的连续侧向磁介电泳微分离器，可高效富集外周血中循环核细胞
3. An integrated enrichment system to facilitate isolation and molecular characterization of single cancer cells from whole blood [J] . Yu Liping, Sa Silin, Wang Ling, Cytometry, Part A: the journal of the International Society for Analytical Cytology . 2018,第12期

机译：一种综合的富集系统，以促进全血单癌细胞的分离和分子表征
4. Detection and Isolation of rare cells by 2-step enrichment high-speed flow cytometry/cell sorting and single cell LEAP laser ablation [C] . Zordan M.D, Leary J.F. Conference on imaging, manipulation, and analysis of biomolecules, cells, and tissues IX . 2011

机译：通过两步富集高速流式细胞仪/细胞分选和单细胞LEAP激光消融检测和分离稀有细胞
5. Impedance Spectroscopy and Dielectrophoretic Activated Cell Sorters (DACS) for Heterogeneous Neural Stem/Progenitor Cells [D] . Lopez Prieto, Javier 2011

机译：异质神经干/祖细胞的阻抗谱和介电激活细胞分选仪（DACS）
6. Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting [O] . D J E Peeters, B De Laere, G G Van den Eynden, 2013

机译：使用Dielectrophoretic细胞分选从CellSearch富集的血液样本中半自动分离单个或高度纯化的肿瘤细胞并进行分子表征
7. Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting [O] . Peeters, D J E, De Laere, B, Van den Eynden, G G, 2013

机译：使用Dielectrophoretic细胞分选从CellSearch富集的血液样本中半自动分离单个或高度纯化的肿瘤细胞并进行分子表征
8. Novel Strategy for Isolation, Molecular and Functional Characterization of Embryonic Mammary Stem Cells Using Molecular Genetics and Microfluidic Sorting [R] . Wahl, G. M. 2008

机译：利用分子遗传学和微流体分选对胚胎乳腺干细胞的分离，分子和功能表征的新策略

Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting

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