Global approaches to quantitative analysis of gene-expression patterns observed by use of two-dimensional gel electrophoresis.

A Gemmell; J P Hofmann; J Taylor; N L Anderson

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Global approaches to quantitative analysis of gene-expression patterns observed by use of two-dimensional gel electrophoresis.

机译：通过二维凝胶电泳观察到的基因表达模式定量分析的整体方法。

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A major difficulty in the use of two-dimensional protein maps to identify and classify cell types is the problem of acquiring, selecting, and analyzing quantitative data on hundreds of protein spots. Here we use methods of multivariate statistics to analyze the differences among a panel of human cell lines, in some cases involving quantitative data on more than 250 proteins. Principal-component and cluster-analysis techniques show that the lines can be easily distinguished, even by using the subset of proteins present in all cells. A preliminary analysis of the protein changes brought about by phorbol ester-induced differentiation of the line U937 is included.

机译：使用二维蛋白质图来识别和分类细胞类型的主要困难是获取，选择和分析数百个蛋白质斑点上定量数据的问题。在这里，我们使用多元统计方法来分析一组人类细胞系之间的差异，在某些情况下，涉及涉及250多种蛋白质的定量数据。主成分和聚类分析技术表明，即使使用所有细胞中存在的蛋白质子集，也可以轻松区分这些品系。包括对佛波醇酯诱导的U937系分化产生的蛋白质变化的初步分析。

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《Clinical Chemistry: Journal of the American Association for Clinical Chemists》 |1984年第12期|共页
作者
A Gemmell; J P Hofmann; J Taylor; N L Anderson;
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中图分类诊断学;
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Global approaches to quantitative analysis of gene-expression patterns observed by use of two-dimensional gel electrophoresis.

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