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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >Fluorescence polarization immunoassay. II. Analyzer for rapid, precise measurement of fluorescence polarization with use of disposable cuvettes.
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Fluorescence polarization immunoassay. II. Analyzer for rapid, precise measurement of fluorescence polarization with use of disposable cuvettes.

机译:荧光偏振免疫测定。二。分析仪,用于使用一次性比色皿进行快速,精确的荧光偏振测量。

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摘要

This instrument, developed for quantitating fluorescence polarization immunoassays, automatically measures both polarization components and computes a polarization value corrected for background and optical bias. An electronically switched liquid crystal provides a nonmechanical means of rotating the plane of polarization in the excitation optics. The electronic design features digital integration and microprocessor controlled functions. Readings are made in disposable 12 X 75 mm round culture tubes. in less than 10 s. Results are precise to 0.001 polarization unit, linear from 10(-7) to 10(-10) mol of fluorescein per liter, and correlate well with those obtained with other "high-performance" instrumentation.
机译:该仪器专为定量荧光偏振免疫测定而开发,可自动测量两个偏振成分并计算针对背景和光学偏差校正的偏振值。电子开关液晶提供了一种非机械方式来旋转激发光学器件中的偏振面。电子设计具有数字集成和微处理器控制的功能。在一次性的12 X 75 mm圆形培养管中读数。少于10秒。结果精确到0.001极化单位,荧光素从每升10(-7)到10(-10)mol呈线性,并且与使用其他“高性能”仪器获得的结果很好相关。

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