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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >Deuterium enrichment of plasma determined by nuclear magnetic resonance spectroscopy: dilution kinetics of 2H2O and estimation of total body water.
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Deuterium enrichment of plasma determined by nuclear magnetic resonance spectroscopy: dilution kinetics of 2H2O and estimation of total body water.

机译：通过核磁共振波谱法测定的血浆氘富集度：2H2O的稀释动力学和体内总水的估算。

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摘要

We demonstrate the feasibility of quantifying the abundance of 2H in plasma by nuclear magnetic resonance (NMR) spectroscopy. After adding internal standard (tert-butyl-d9 alcohol) to deproteinized plasma samples containing 2H2O, we determined the ratio of NMR peak areas for 2H2O and tert-butyl-d9 alcohol. This peak-area ratio was directly proportional to the exogenous 2H enrichment of plasma (difference between measured and naturally occurring 2H) between 0 and 0.272 atom % (r = 0.999). The coefficient of variation was 1.34% at an exogenous enrichment of 0.136 atom %. We applied this method to a study of the dilution kinetics of 2H2O to determine the optimal time and method of blood sampling for estimation of total body water content. The 2H enrichment of plasma stabilized by 4 h after intravenous injection of 2H2O, 1 g/kg of body weight, and fluctuated within 2-4% of the 4- to 8-h mean thereafter.
机译：我们证明了通过核磁共振（NMR）光谱定量血浆中2 H丰度的可行性。在将内标（叔丁基-d9醇）添加到含有2H2O的去蛋白血浆样品中后，我们确定2H2O和叔丁基-d9醇的NMR峰面积比。该峰面积比与血浆中外源2H富集成正比（测得的2H与天然存在的2H之差）介于0和0.272原子％之间（r = 0.999）。在0.136原子％的外源富集下，变异系数为1.34％。我们将此方法应用于2H2O稀释动力学的研究，以确定最佳的时间和方法来估计人体总含水量。静脉注射2H2O，1 g / kg体重后4 h，血浆2H富集稳定，随后在4至8h平均值的2-4％范围内波动。

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