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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >A commercial enzyme immunoassay method (EMIT) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol.
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A commercial enzyme immunoassay method (EMIT) compared with liquid chromatography and bioassay methods for measurement of chloramphenicol.

机译：商业酶免疫测定法（EMIT）与液相色谱法和生物测定法相比较，用于测定氯霉素。

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摘要

A new enzyme immunoassay method (EMIT; Syva Co.) was compared with conventional high-performance liquid chromatography (HPLC) and agar-diffusion bioassay methods for measurement of chloramphenicol in human serum. Forty-nine serum samples were assayed by each of the three methods. Excellent correlation was observed between values by EMIT and by the two conventional methods (r = 0.986 and 0.961). Precision was acceptable (CV less than 5%) with EMIT. Assay of samples containing chloramphenicol glucuronide and chloramphenicol succinate demonstrated that EMIT recognizes only the biologically active (base) form of the drug. The capability to test serum samples as small as 0.2 mL, adaptation to widely available instrumentation, and provision of rapid results are principal advantages of the EMIT method for routine chloramphenicol measurements.
机译：比较了一种新的酶免疫分析方法（EMIT; Syva Co.）与传统的高效液相色谱法（HPLC）和琼脂扩散生物分析方法来测定人血清中的氯霉素。通过这三种方法分别测定了49个血清样品。通过EMIT和两种常规方法观察到的值之间具有极好的相关性（r = 0.986和0.961）。 EMIT的精度是可以接受的（CV小于5％）。包含氯霉素葡萄糖醛酸苷和琥珀酸氯霉素的样品的分析表明，EMIT仅识别该药物的生物活性（碱）形式。 EMIT方法用于常规氯霉素测量的主要优点是能够检测低至0.2 mL的血清样品，适应广泛使用的仪器并提供快速的结果。

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