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首页> 外文期刊>Applied and Environmental Microbiology >Dissimilatory nitrate reduction to nitrate, nitrous oxide, and ammonium by Pseudomonas putrefaciens.
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Dissimilatory nitrate reduction to nitrate, nitrous oxide, and ammonium by Pseudomonas putrefaciens.

机译：腐烂假单胞菌将硝酸盐异化为硝酸盐，一氧化二氮和铵。

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摘要

The influence of redox potential on dissimilatory nitrate reduction to ammonium was investigated on a marine bacterium, Pseudomonas putrefaciens. Nitrate was consumed (3.1 mmol liter-1), and ammonium was produced in cultures with glucose and without sodium thioglycolate. When sodium thioglycolate was added, nitrate was consumed at a lower rate (1.1 mmol liter-1), and no significant amounts of nitrite or ammonium were produced. No growth was detected in glucose media either with or without sodium thioglycolate. When grown on tryptic soy broth, the production of nitrous oxide paralleled growth. In the same medium, but with sodium thioglycolate, nitrous oxide was first produced during growth and then consumed. Acetylene caused the nitrous oxide to accumulate. These results and the mass balance calculations for different nitrogen components indicate that P. putrefaciens has the capacity to dissimilate nitrate to ammonium as well as to dinitrogen gas and nitrous oxide (denitrification). The dissimilatory pathway to ammonium dominates except when sodium thioglycolate is added to the medium.
机译：在海洋细菌Pseudomonas putrefaciens上研究了氧化还原电势对异化硝酸盐还原为铵的影响。消耗了硝酸盐（3.1 mmol升-1），在有葡萄糖和无硫代乙醇酸钠的培养物中产生了铵。当加入巯基乙酸钠时，硝酸盐的消耗速率较低（1.1 mmol升-1），并且不会产生大量的亚硝酸盐或铵盐。在有或没有巯基乙酸钠的葡萄糖培养基中均未检测到生长。当在胰蛋白酶大豆肉汤中生长时，一氧化二氮的产生与生长平行。在相同的培养基中，但使用巯基乙酸钠，首先在生长过程中产生一氧化二氮，然后消耗。乙炔导致一氧化二氮积累。这些结果和针对不同氮成分的质量平衡计算表明，腐烂假单胞菌具有将硝酸盐异构化为铵，以及将氮气分解为二氧化氮和一氧化二氮的能力（反硝化作用）。铵的异化途径占主导地位，除非将巯基乙酸钠添加到培养基中。

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