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首页> 外文期刊>Applied and Environmental Microbiology >Bacteria isolated from the duodenum, ileum, and cecum of young chicks.
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Bacteria isolated from the duodenum, ileum, and cecum of young chicks.

机译:从小鸡的十二指肠,回肠和盲肠分离出的细菌。

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Facultatively anaerobic and strictly anaerobic bacteria colonizing the intestinal tracts of 14-day-old chicks fed a corn-based diet were enumerated, isolated, and identified. Colony counts from anaerobic roll tubes (rumen fluid medium) or aerobic plates (brain heart infusion agar) recovered from homogenates of the duodenum, upper and lower ileum, and cecum varied appreciably among samples from individual birds. Anaerobic and aerobic counts from the duodenum and ileum were similar. Anaerobic counts were highest from the cecum (0.7 X 10(11) to 1.6 X 10(11)/g of dry tissue) and exceeded aerobic plate counts by a factor of at least 10(2). Facultatively anaerobic groups (Streptococcus, Staphylococcus, Lactobacillus, and Escherichia coli) comprised the predominant flora of the duodenum and ileum, although large numbers of anaerobes (9 to 39% of the small intestine isolates), represented by species of Eubacterium, Propionibacterium, Clostridium, Gemmiger, and Fusobacterium, were also recovered. Strict anaerobes (anaerobic gram-positive cocci, Eubacterium, Clostridium Gemmiger, Fusobacterium, and Bacteriodes) made up nearly the entire microbial population of the cecum. Scanning electron microscopy of the intestinal epithelia of chicks revealed populations of microbes on the duodenal, ileal, and cecal mucosal surfaces.
机译:列举,分离和鉴定了以玉米饮食喂养的14日龄雏鸡肠道中的兼性厌氧菌和严格厌氧菌。从十二指肠,回肠上,下回肠和盲肠匀浆中回收的厌氧转管(瘤胃液培养基)或需氧板(脑心浸液琼脂)的菌落数在个别鸟类的样本中明显不同。十二指肠和回肠的无氧和有氧计数相似。盲肠的厌氧菌数最高(0.7 X 10(11)至1.6 X 10(11)/克干组织),超过需氧菌板数至少10(2)。兼性厌氧菌(链球菌,葡萄球菌,乳杆菌和大肠杆菌)构成了十二指肠和回肠的主要菌群,尽管大量的厌氧菌(占小肠分离株的9%至39%)以真细菌,丙酸杆菌,梭菌为代表。 ,Gemmiger和Fusobacterium也被回收。严格的厌氧菌(厌氧革兰氏阳性球菌,Eubacterium,Gemmiger梭状芽孢杆菌,Fusobacterium和Bacteriodes)几乎构成了盲肠的整个微生物种群。雏鸡肠上皮的扫描电子显微镜显示十二指肠,回肠和盲肠粘膜表面有微生物种群。

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