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首页> 外文期刊>Applied and Environmental Microbiology >Effect of aeration and carbon dioxide on cell morphology of Candida utilis.
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Effect of aeration and carbon dioxide on cell morphology of Candida utilis.

机译：曝气和二氧化碳对假丝酵母细胞形态的影响。

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The effect of CO2 availability on cell size, shape, and aggregation in continuous cultures of Candida utilis was studied in minimal medium with glucose or ethanol as the sole carbon and energy source. Enrichment with CO2 was achieved (i) by using the substrate with more C atoms, (ii) by using pure oxygen and thus decreasing aeration intensity at the same dissolved-oxygen concentration, or (iii) by adding CO2 to the aeration gas. The cells were always of yeast shape, and no filaments were formed. In cultures with a biomass concentration above 6 g (dry weight) per liter, no cell aggregates were observed. In cultures with a lower biomass, the daughter cells failed to separate from the parent cells and formed aggregates with thickened walls. The average cell number per aggregate was found to be higher, and the average protoplast volume lower, under conditions of probable CO2 limitation. Simultaneously, the ratio of total dry weight to wet weight of protoplasts was considerably higher, indicating an increased share of wall or extracellular material. The possible effect of the observed morphological changes for maintaining a suitable concentration gradient of CO2 around the cell is discussed.
机译：在以葡萄糖或乙醇为唯一碳源和能源的基本培养基中，研究了可利用二氧化碳对连续使用假丝酵母的细胞大小，形状和聚集的影响。 （i）通过使用具有更多C原子的底物，（ii）通过使用纯氧并因此在相同的溶解氧浓度下降低曝气强度来实现CO2的富集，或（iii）通过向曝气气体中添加CO2来实现。细胞始终是酵母形状的，没有丝状形成。在每升生物质浓度超过6 g（干重）的培养物中，未观察到细胞聚集体。在具有较低生物量的培养物中，子细胞未能与亲本细胞分离，并形成壁增厚的聚集体。发现在可能的CO 2限制条件下，每个聚集体的平均细胞数更高，平均原生质体体积更低。同时，原生质体的总干重与湿重之比明显更高，表明壁或细胞外材料的份额增加。讨论了观察到的形态变化对维持细胞周围合适的CO2浓度梯度的可能影响。

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