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首页> 外文期刊>Applied and Environmental Microbiology >Interaction of Pseudomonas solanacearum with Suspension-Cultured Tobacco Cells and Tobacco Leaf Cell Walls In Vitro
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Interaction of Pseudomonas solanacearum with Suspension-Cultured Tobacco Cells and Tobacco Leaf Cell Walls In Vitro

机译：青枯假单胞菌与悬浮培养的烟草细胞和烟草叶细胞壁的相互作用

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摘要

Attachment of radiolabeled Pseudomonas solanacearum cells to suspension-cultured tobacco cells and tobacco leaf cell walls was measured in vitro by a filtration technique that allowed separation of attached and unattached bacteria. An avirulent strain (B1) attached more rapidly to suspension-cultured cells than did the virulent parent strain (K60), and B1 attachment was less sensitive to inhibition by high ionic strength than was K60. Attachment of B1 bacteria to suspension-cultured cells and to leaf cell walls was comparable (50 to 70%), but only a small proportion (10 to 20%) of K60 bacteria attached to leaf cell walls under optimal conditions. With high bacterial populations (108 bacteria per ml), attachment of K60 to suspension-cultured cells was greatly reduced. Attachment of both strains was completely inhibited by pretreating bacterial cells with heat (41°C) or azide and was partially inhibited by EDTA and kanamycin. The mechanism of attachment is not known, but ionic forces may be involved.
机译：通过允许分离附着细菌和未附着细菌的过滤技术，在体外测量了放射性标记的青枯假单胞菌细胞与悬浮培养的烟草细胞和烟草叶细胞壁的附着。无毒力的菌株（B1）比有毒的亲代菌株（K60）附着在悬浮培养细胞上的速度更快，而B1的附着对高离子强度抑制的敏感性比K60差。 B1细菌在悬浮培养细胞和叶细胞壁上的附着率相当（50％至70％），但是在最佳条件下，只有60％的K60细菌在叶细胞壁上附着。随着细菌数量的增加（每毫升108个细菌），K60与悬浮培养细胞的附着大大减少。通过加热（41°C）或叠氮化物预处理细菌细胞，两种菌株的附着均被完全抑制，而EDTA和卡那霉素则部分抑制了它们的附着。附着的机制尚不清楚，但可能会涉及离子力。

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