...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Microcomputer-Assisted Biomass Determination of Plankton Bacteria on Scanning Electron Micrographs
【24h】

Microcomputer-Assisted Biomass Determination of Plankton Bacteria on Scanning Electron Micrographs

机译：扫描电子显微镜微机辅助浮游生物的生物量测定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although biovolume is a better measure of biomass than is cell number, biovolumes have rarely been measured because their evaluation is extremely time-consuming. We developed a microcomputer system that assists cell size measurements on images of filtered plankton: scanning electron micrograph negatives were projected on a digitizer field, bacterial length and width were marked by a cursor, and coordinates were directly transferred to an MOS 6502 microcomputer (KIM 1). The dialogue program BABI organized and controlled the digitizer measurements in cooperation with the user, enabled corrections, and printed out results with 95% confidence limits and sample description. The time for scanning electron micrograph preparation was reduced to 15 min (quick transfer to Freon 113 during filtration and air drying). Altogether, this biovolume determination took about 2.5 h for confidence limits of ±15%. Examples are given for applications of the method: (i) comparison of 10 lakes (with specific activities for glucose uptake and for heterotrophic CO2 fixation); (ii) ranges of biomass parameters in one lake; (iii) diurnal cycles (with synchronizing effects, uptake of algal exudates, and calculation of daily growth). This method is discussed in relation to other biomass methods (epifluorescent microscopy, lipopolysaccharide technique, frequency of dividing cells) and the problem of biovolume-to-carbon conversions.
机译：尽管生物量比细胞数量更好地衡量了生物量，但是生物量很少被测量，因为它们的评估非常耗时。我们开发了一种微型计算机系统，该系统可帮助对过滤后的浮游生物的图像进行细胞大小测量：将扫描电子显微图的负片投影到数字化仪场上，用光标标记细菌的长度和宽度，并将坐标直接传输到MOS 6502微型计算机（KIM 1 ）。对话程序BABI与用户合作组织和控制了数字化仪的测量，进行了更正，并打印出具有95％置信度限制和样品描述的结果。扫描电子显微图的准备时间减少到15分钟（在过滤和空气干燥过程中迅速转移到Freon 113）。总的来说，此生物量测定花费了约2.5小时，置信限为±15％。举例说明了该方法的应用：（i）比较10个湖泊（具有特定的葡萄糖摄取和固定异养二氧化碳的活性）; （ii）一个湖泊的生物量参数范围; （iii）昼夜周期（具有同步效应，藻类渗出物的吸收和日生长的计算）。讨论了其他生物质方法（荧光显微镜，脂多糖技术，分裂细胞的频率）以及生物量向碳转化的问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号