Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO.

W Jarausch; C Saillard; F Dosba; J M Bové

首页> 外文期刊>Applied and Environmental Microbiology >Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO.

【24h】

Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO.

机译：通过使用源自苹果繁殖MLO染色体片段序列的特异性引物进行PCR，在欧洲果树中区分支原体样生物（MLO）。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. The protein encoded by open reading frame 2 shows significant homologies with bacterial nitroreductases. From the nucleotide sequence four primer pairs for PCR were chosen to specifically amplify DNA from MLOs associated with European diseases of fruit trees. Primer pairs specific for (i) Malus-affecting MLOs, (ii) Malus- and Prunus-affecting MLOs, and (iii) Malus-, Prunus-, and Pyrus-affecting MLOs were obtained. Restriction enzyme analysis of the amplification products revealed restriction fragment length polymorphisms between Malus-, Prunus, and Pyrus-affecting MLOs as well as between different isolates of the apple proliferation MLO. No amplification with either primer pair could be obtained with DNA from 12 different MLOs experimentally maintained in periwinkle.

机译：对与苹果繁殖相关的支原体样生物（MLO）的一个1.8-kb染色体DNA片段进行了测序。在该片段上观察到三个推定的开放阅读框。由开放阅读框2编码的蛋白质显示出与细菌硝基还原酶的显着同源性。从核苷酸序列中选择四个用于PCR的引物对，以特异性扩增来自与欧洲果树疾病相关的MLO的DNA。获得了特异于（i）影响苹果属的MLO，（ii）影响苹果属和李的MLO，以及（iii）影响苹果属，李子和Pyrus的MLO的引物对。扩增产物的限制性酶分析显示，在影响苹果的Malus，Prunus和Pyrus的MLO之间以及苹果增殖MLO的不同分离株之间，存在限制性片段长度多态性。用实验上保持在长春花中的12种不同MLO的DNA不能获得任一引物对的扩增。

著录项

来源
《Applied and Environmental Microbiology》 |1994年第8期|共8页
作者
W Jarausch; C Saillard; F Dosba; J M Bové;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类植物学;
关键词

相似文献

外文文献
专利

1. Genetic Interrelatedness among Clover Proliferation Mycoplasmalike Organisms (MLOs) and Other MLOs Investigated by Nucleic Acid Hybridization and Restriction Fragment Length Polymorphism Analyses [J] . Ing-Ming Lee, Robert E. Davis, Chuji Hiruki Applied and Environmental Microbiology . 1991,第12期

机译：三叶草扩散支原体生物（MLOs）和其他MLOs之间的遗传相关性通过核酸杂交和限制性片段长度多态性分析研究
2. Differentiation of pathogenic and nonpathogenic isolates of Geotrichum candidum sensu Suprapta et al. (1995) on citrus fruit based on PCR-RFLP analysis of rDNA ITS and PCR using specific primers designed in polygalacturonase genes [J] . Nakamura Masayuki, Suprapta Dewa Ngurah, Iwai Hisashi Mycoscience . 2008,第2期

机译：念珠菌致病性和非致病性分离株的分化Suprapta等。（1995）在柑橘类水果上进行了基于rDNA ITS的PCR-RFLP分析，并使用了在聚半乳糖醛酸酶基因中设计的特异性引物进行PCR
3. Differentiation of pathogenic and nonpathogenic isolates of Geotrichum candidum sensu Suprapta et al. (1995) on citrus fruit based on PCR-RFLP analysis of rDNA ITS and PCR using specific primers designed in polygalacturonase genes [J] . Masayuki Nakamura, Dewa Ngurah Suprapta, Hisashi Iwai Mycoscience . 2008,第2期

机译：念珠菌致病性和非致病性分离株的分化Suprapta等。（1995）基于rDNA ITS的PCR-RFLP分析和使用在聚半乳糖醛酸酶基因中设计的特异性引物进行PCR的柑橘类水果
4. Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO. [O] . W Jarausch, C Saillard, F Dosba, 1994

机译：通过使用源自苹果繁殖MLO染色体片段序列的特异性引物进行PCR在欧洲果树中区分支原体样生物（MLO）。
5. Differentiation of virescence MLOs using western aster yellows mycoplasma-like organism chromosomal DNA probes and restriction fragment length polymorphism analysis [O] . Cheryl R. Kuske, Bruce C. Kirkpatrick, Erich Seemüller 1991

机译：使用Western Aster yellows mycoplasma的生物体染色体DNA探针和限制片段长度多态性分析

Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO.

摘要

著录项

相似文献

相关主题

期刊订阅