Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.

S W Hooper; T C Dockendorff; G S Sayler

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Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.

机译：4-氯联苯分解代谢质粒pSS50的特征和限制性分析。

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The plasmid pSS50 is a 53-kilobase self-transmissible plasmid of broad host range that has been isolated from several Alcaligenes and Acinetobacter species. This plasmid has previously been shown to mediate the mineralization of 4-chlorobiphenyl to carbon dioxide and water. Physical characterization of this plasmid by restriction analysis indicates that most hexanucleotide cleavage sites are clustered in a 5-kilobase region, leaving large regions without restriction sites. The paucity of restriction sites is not due to DNA methylation.

机译：质粒pSS50是一种具有53个碱基的自我传播质粒，具有广泛的宿主范围，已从几种产碱杆菌和不动杆菌中分离出来。先前已证明该质粒介导4-氯联苯矿化成二氧化碳和水。通过限制性酶切分析对该质粒的物理特征表明，大多数六核苷酸切割位点聚集在5-碱基的区域，而大的区域没有限制性位点。限制性位点的缺乏不是由于DNA甲基化。

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《Applied and Environmental Microbiology》 |1989年第5期|共3页
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S W Hooper; T C Dockendorff; G S Sayler;
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Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50.

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