Conjugative Plasmid in Corynebacterium flaccumfaciens subsp. oortii That Confers Resistance to Arsenite, Arsenate, and Antimony(III)

Carol A. Hendrick; William P. Haskins; Anne K. Vidaver

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Conjugative Plasmid in Corynebacterium flaccumfaciens subsp. oortii That Confers Resistance to Arsenite, Arsenate, and Antimony(III)

机译：flaccumfaciens亚种棒状杆菌中的结合质粒。赋予对亚砷酸盐，砷酸盐和锑的抗性的oortii（III）

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Gene transfer systems for phytopathogenic corynebacteria have not been reported previously. In this paper we describe a conjugative 46-megadalton plasmid (pDG101) found in Corynebacterium flaccumfaciens subsp. oortii CO101 that mediates resistance to arsenite, arsenate, and antimony(III). Transfer of the plasmid from CO101 to four other strains from the C. flaccumfaciens group occurred between cells immobilized on nitrocellulose filters or on agar surfaces. Transconjugant strains expressed the same levels of metal resistance as the donor strain and were able to act as donor strains in subsequent matings. The physical presence of the plasmid was detected by agarose gel electrophoresis. Arsenite-sensitive derivatives of the donor and transconjugant strains were obtained after heat treatment; these were cured of pDG101.

机译：以前没有关于植物致病性棒杆菌基因转移系统的报道。在本文中，我们描述了在黄杆菌棒状杆菌亚种中发现的46兆道尔顿共轭质粒（pDG101）。 oortii CO101，介导对亚砷酸盐，砷酸盐和锑的抗性（III）。将质粒从CO101转移到黄曲霉组的其他四个菌株中，这发生在固定于硝酸纤维素滤膜或琼脂表面的细胞之间。转结合菌株表现出与供体菌株相同的金属抗性水平，并且能够在随后的交配中充当供体菌株。通过琼脂糖凝胶电泳检测质粒的物理存在。热处理后获得供体和转结合体菌株的砷敏感衍生物。这些被pDG101治愈。

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《Applied and Environmental Microbiology》 |1984年第1期|共5页
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Carol A. Hendrick; William P. Haskins; Anne K. Vidaver;
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1. Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (III) in escherichia coli and Staphylococcus aureus. [J] . S Silver, K Budd, K M Leahy, Journal of bacteriology . 1981,第3期

机译：诱导型质粒确定了大肠杆菌和金黄色葡萄球菌对砷酸盐，亚砷酸盐和锑（III）的抗性。
2. A Transferable 20-Kilobase Multiple Drug Resistance-Conferring R Plasmid (pKL0018) from a Fish Pathogen (Lactococcus garvieae) Is Highly Homologous to a Conjugative Multiple Drug Resistance-Conferring Enterococcal Plasmid [J] . Takeshi Maki, Mudjekeewis D. Santos, Hidehiro Kondo, Applied and Environmental Microbiology . 2009,第10期

机译：来自鱼病原体（乳乳球菌）的可转移的20碱基多重耐药性R质粒（pKL0018）与共轭多重耐药性肠球菌质粒高度同源
3. A Transferable 20-Kilobase Multiple Drug Resistance-Conferring R Plasmid (pKL0018) from a Fish Pathogen (Lactococcus garvieae) Is Highly Homologous to a Conjugative Multiple Drug Resistance-Conferring Enterococcal Plasmid [J] . Takeshi Maki, Mudjekeewis D. Santos, Hidehiro Kondo, Applied and Environmental Microbiology . 2009,第10期

机译：来自鱼病原体（乳乳球菌）的可转移的20碱基多重耐药性R质粒（pKL0018）与共轭多重耐药性肠球菌质粒高度同源
4. Adsorption of Arsenite(III) and Arsenate(V) from Drinking Water Using Amorphous and Anatase Titanium Dioxide as the Adsorbent [C] . Yuchao Tang, Xianhuai Huang, Lihua Tang, International Conference on Green Building, Materials and Civil Engineering . 2011

机译：使用无定形和锐钛矿二氧化物作为吸附剂，吸附砷酸盐（III）和砷酸酯（v）
5. Adsorption and redox transformation of arsenite (As(III)) and arsenate (As(V)) at water-mineral interfaces. [D] . Sun, Xiaohua. 1998

机译：在水-矿物界面处吸附和氧化还原砷（As（III））和砷酸盐（As（V））。
6. Conjugative Plasmid in Corynebacterium flaccumfaciens subsp. oortii That Confers Resistance to Arsenite Arsenate and Antimony(III) [O] . Carol A. Hendrick, William P. Haskins, Anne K. Vidaver 1984

机译：flaccumfaciens亚种棒状杆菌中的结合质粒。赋予对亚砷酸亚砷酸和锑的抗性的oortii（III）
7. Reclassification of Corynebacterium flaccumfaciens, Corynebacterium betae, Corynebacterium oortii and Corynebacterium poinsettiae in the genus Curtobacterium, as Curtobacterium flaccumfaciens comb. nov. [O] . M. D. Collins, D. Jones 1983

机译：重新分类Flaccumfaciens，CoryneBacterium Betae，Corecacterium属的糖尿病，卵石菌和棒状杆菌一品红，如凝胶杆菌Flaccumfaciens梳状。 11月。

Conjugative Plasmid in Corynebacterium flaccumfaciens subsp. oortii That Confers Resistance to Arsenite, Arsenate, and Antimony(III)

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