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首页> 外文期刊>Applied and Environmental Microbiology >Horizontal Transfer of the Plant Virulence Gene, nec1, and Flanking Sequences among Genetically Distinct Streptomyces Strains in the Diastatochromogenes Cluster
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Horizontal Transfer of the Plant Virulence Gene, nec1, and Flanking Sequences among Genetically Distinct Streptomyces Strains in the Diastatochromogenes Cluster

机译：Diastatochromogenes群集中遗传上不同的链霉菌菌株之间的植物毒力基因，nec1和侧翼序列的水平转移。

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摘要

Evidence for the horizontal transfer of a pathogenicity island (PAI) carrying the virulence gene nec1 and flanking sequences among Streptomyces strains in the Diastatochromogenes cluster is presented. Plant-pathogenic, thaxtomin-producing Streptomyces strains, previously classified as S. scabiei based on the conventionally used phenotypic characteristics, were found to be genetically distinct from the type strain of S. scabiei based on DNA relatedness and 16S rDNA sequence analysis. Pairwise DNA-DNA hybridizations between some of these strains and the S. scabiei type strain were as low as 36%, a value much below what is conventionally accepted for species identity (70%). The sequence of the nec1 gene, however, was identical in all the S. scabiei and S. scabiei-like strains tested, irrespective of their DNA relatedness to the type strain of S. scabiei, their geographic origin, or the isolation host. Furthermore, a 26-kb DNA fragment including and flanking nec1 was also conserved among these strains based on restriction and Southern analyses. These data indicate that the etiology of potato scab is more complex than previously recognized; this result has important implications for potato scab management strategies. Previous research has suggested that horizontal transfer of a PAI was the mechanism for evolution of pathogenicity in S. acidiscabies and S. turgidiscabies, species that lie outside of the Diastatochromogenes cluster. Data presented here support this model and indicate that PAI transfer also has occurred frequently in species closely related to S. scabiei.
机译：提出了在Diastatochromogenes集群中链霉菌菌株中带有毒力基因nec1和侧翼序列的致病岛（PAI）水平转移的证据。基于DNA相关性和16S rDNA序列分析，发现植物致病性产thax​​tomin的链霉菌菌株，根据常规使用的表型特征先前被分类为S. scabiei，在遗传上不同于S. scabiei。这些菌株中的一些与S. scabiei型菌株之间的成对DNA-DNA杂交率低至36％，远低于传统上公认的物种同一性（70％）。但是，nec1基因的序列在所测试的所有S. scabiei和S. scabiei菌株中都是相同的，而不管它们与S. scabiei菌株的DNA相关性，地理来源或分离宿主如何。此外，基于限制性酶切和Southern分析，在这些菌株中还保留了包含和侧翼nec1的26kb DNA片段。这些数据表明，马铃薯sc的病因比以前认识的要复杂。这一结果对马铃薯sc的管理策略具有重要意义。先前的研究表明，PAI的水平转移是嗜酸性链球菌和turgidiscabies链球菌（位于Diastatochromogenes簇外的物种）致病性进化的机制。这里提供的数据支持该模型，并表明PAI转移也经常发生在与S. scabiei密切相关的物种中。

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